Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 16630548Journal NLM ID: 0372516Publisher: Academic Press
Correspondence: wang.892
osu.edu
Institutions: Departments of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA
O-repeating unit biosynthesis is the first committed step in lipopolysaccharide (LPS) biosynthesis in a variety of gram-negative bacteria. The wbnH gene was previously proposed to encode a glycosyltransferase involved in O-repeating unit synthesis in Escherichia coli O86:H2 strain. In this work, we provide biochemical evidence to show that wbnH encodes a N-acetylgalactosaminyl transferase (GalNAcT) that catalyzes the transfer of GalNAc from UDP-GalNAc to the GalNAc-pyrophosphate-lipid acceptor. WbnH activity was characterized using a synthetic acceptor substrate GalNAcα-PP-O(CH(2))(11)-OPh. The resulting disaccharide product GalNAc-α-1,3-GalNAcα-PP-O(CH(2))(11)-OPh was analyzed by LC-MS and NMR spectroscopy. Substrate specificity study indicates that pyrophosphate and hydrophobic lipid moiety are structural requirements for WbnH activity. Divalent metal cations are not required for enzyme catalysis, suggesting WbnH belongs to glycosyltransferase GT-B superfamily. Our results complete the characterization of O86 O-unit assembly pathway, and provide the access of chemically defined O-unit substrates for the further investigation of O-antigen biosynthetic mechanism
glycosyltransferase, O-Repeating unit, O-antigen biosynthesis, Undecaprenyl phosphate, wzy-dependent
Structure type: suggested polymer biological repeating unit
Location inside paper: p.632,fig.2
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_130648,IEDB_134627,IEDB_136044,IEDB_136045,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_1391961,IEDB_1391963,IEDB_140125,IEDB_141582,IEDB_141584,IEDB_141794,IEDB_142489,IEDB_143260,IEDB_144562,IEDB_149558,IEDB_150766,IEDB_150948,IEDB_150952,IEDB_151528,IEDB_152212,IEDB_152214,IEDB_153207,IEDB_153553,IEDB_174333,IEDB_190606,IEDB_241096,IEDB_461710,IEDB_461718,IEDB_461719,IEDB_885822,IEDB_918314,SB_148,SB_154,SB_165,SB_166,SB_187,SB_195,SB_23,SB_24,SB_7,SB_8,SB_86,SB_87,SB_88
Methods: NMR
Enzymes that release or process the structure: GlcNAc transferase
Biosynthesis and genetic data: genetic data, biosynthetic data
Related record ID(s): 10035, 10195, 10548, 20043, 20405, 20406, 20407, 20408, 20409, 20508, 20667, 25273, 108706, 115586
NCBI Taxonomy refs (TaxIDs): 2162909Reference(s) to other database(s): GTC:G75734HT, GlycomeDB:
3571
Show glycosyltransferases
There is only one chemically distinct structure:
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