Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: cystic fibrosis (CF) [ICD11:
CA25 
]
NCBI PubMed ID: 16325774Publication DOI: 10.1016/j.bbrc.2005.11.104Journal NLM ID: 0372516Publisher: Academic Press
Correspondence: cescutti
bbcm.units.it
Institutions: Dipartimento di Biochimica Biofisica e Chimica delle Macromolecole, Università di Trieste, via L. Giorgieri 1, I-34127 Trieste, Italy
Bacteria belonging to the Burkholderia cepacia complex (Bcc) are interesting for their involvement in pulmonary infections in patients affected by cystic fibrosis (CF) or chronic granulomatous disease. Many Bcc strains isolated from CF patients produce high amounts of exopolysaccharides (EPS). Although different strains sometimes biosynthesise different EPS, the majority of Bcc bacteria produce only one type of polysaccharide, which is called cepacian. The polymer has a unique heptasaccharidic repeating unit, containing three side chains, and up to three O-acetyl substituents.. We here report for the first time the isolation and characterisation of a lyase active towards cepacian produced by a Bacillus sp., which was isolated in our laboratory. The enzyme molecular mass, evaluated by size-exclusion chromatography, is 32,700+/-1500Da. The enzyme catalyses a beta-elimination reaction of the disaccharide side chain β-D-Galp-(1→2)-α-D-Rhap-(1→ from the C-4 of the glucuronic acid residue present in the polymer backbone. Although active on both native and de-acetylated cepacian, the enzyme showed higher activity on the latter polymer.
NMR spectroscopy, cystic fibrosis, cepacian, Burkholderia cepacia complex, Bacillus sp., Polysaccharide lyase
Structure type: polymer chemical repeating unit
Location inside paper: p.822, fig. 1
Trivial name: cepacian
Compound class: EPS
Contained glycoepitopes: IEDB_115136,IEDB_130701,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_1394181,IEDB_140630,IEDB_141794,IEDB_142488,IEDB_144983,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_983930,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_44,SB_67,SB_7,SB_72,SB_88
Methods: 1H NMR, ESI-MS, biochemical methods
Enzymes that release or process the structure: polysaccharide lyase
Synthetic data: enzymatic
Comments, role: lyase specific for cepacian, produced by a Bacillus sp.
Related record ID(s): 1207, 3154, 4338, 7433, 10142, 10158, 10790, 21560, 22655, 25927
NCBI Taxonomy refs (TaxIDs): 87882Reference(s) to other database(s): GTC:G16935RB, GlycomeDB:
25352
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There is only one chemically distinct structure:
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(previously named: Pseudomonas cepacia complex)