Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Oreochromis niloticus niloticus
The structure was elucidated in this paperNCBI PubMed ID: 16759227Publication DOI: 10.1111/j.1742-4658.2006.05311.xJournal NLM ID: 101229646Publisher: Blackwell Publishing
Correspondence: evguenii.vinogradov
nrc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada, Department of Biochemistry and Microbiology, University of Victoria, BC, Canada, Carlsberg Laboratory, Copenhagen, Denmark
Lipopolysaccharide (LPS) and b-glucan from Francisella victoria, a fish pathogen and close relative of highly virulent mammal pathogen Francisella tularensis, have been analyzed using chemical and spectroscopy methods. The polysaccharide part of the LPS was found to contain a nonrepetitive sequence of 20 monosaccharides as well as alanine, 3-aminobutyric acid, and a novel branched amino acid, thus confirming F. victoria as a unique species. The structure identified composes the largest oligosaccharide elucidated by NMR so far, and was possible to solve using high field NMR with cold probe technology combined with the latest pulse sequences, including the first application of H2BC sequence to oligosaccharides. The nonphosphorylated lipid A region of the LPS was identical to that of other Francisellae, although one of the lipid A components has not been found in Francisella novicida. The heptoseless core-lipid A region of the LPS contained a linear pentasaccharide fragment identical to the corresponding part of F. tularensis and F. novicida LPSs, differing in side-chain substituents. The linkage region of the O-chain also closely resembled that of other Francisella. LPS preparation contained two characteristic glucans, previously observed as components of LPS preparations from other strains of Francisella: amylose and the unusual b-(1-6)-glucan with (glycerol)2phosphate at the reducing end.
core, lipid A, O-chain, Francisella, Francisella victoria, lipopolysacchride
Structure type: polymer chemical repeating unit ; n>1
Location inside paper: p.3005
Aglycon: xDGro(1-P-1)x?Gro
Compound class: LOS
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: NMR, ESI-MS, MALDI-MS, composition analysis
Comments, role: linked to position 3 of xDGro(1-P-1)x?Gro. host organism is fish Tilapia
Related record ID(s): 20039, 20318, 20320, 20321, 20322, 20426
NCBI Taxonomy refs (TaxIDs): 262Reference(s) to other database(s): GTC:G26777BZ, GlycomeDB:
863
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There is only one chemically distinct structure:
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