Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Campylobacter jejuni [ICD11:
XN4Q5 
]
NCBI PubMed ID: 16978359Journal NLM ID: 7705721Publisher: Blackwell Publishing
Correspondence: angela.savage
nuigalway.ie
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Department of Microbiology, National University of Ireland, Galway, Ireland, Department of Chemistry, National University of Ireland, Galway, Ireland
The nature of the polysaccharide molecules of the human enteric pathogen Campylobacter jejuni has been the subject of debate. Previously, C. jejuni 81116 was shown to contain two different polysaccharides, one acidic (polysaccharide A) and the other neutral (polysaccharide B), occurring in a 3 : 1 ratio, respectively. The aim of this study was to determine the molecular origin of these polysaccharides. Using a combination of centrifugation, gel permeation chromatography, chemical assays, and (1)H-NMR analysis, polysaccharide B was shown to be derived from lipopolysaccharide and polysaccharide A from capsular polysaccharide. Thus, C. jejuni 81116 produces both lipopolysaccharide-like molecules and capsular polysaccharide.
Lipopolysaccharide, capsular polysaccharide, Campylobacter jejuni, lipo-oligosaccharide, chemical separation.
Structure type: polymer chemical repeating unit
Location inside paper: p.215, fig.1, polysaccharide B
Trivial name: neutral polysaccharide
Compound class: CPS, O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_983931,SB_173,SB_192,SB_7
Methods: methylation, NMR, sugar analysis
Biological activity: serological data
Related record ID(s): 272, 20076, 108718, 114538, 122941
NCBI Taxonomy refs (TaxIDs): 197Reference(s) to other database(s): GTC:G20980KH, GlycomeDB:
3577
Show glycosyltransferases
NMR conditions: in D2O at 333(H) K
[as TSV]
13C NMR data:
missing...
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4 aDGlcp 4.95 3.48 3.70 3.46 4.26 3.95-4.16
3,3,2 Ac - 2.03
3,3 bDGlcpN 4.65 3.62 3.58 3.44 3.44 3.77-3.94
3 aDGalp 5.46 3.96 3.85 4.28 3.94 3.76-3.81
2 Ac - 2.08
bDGlcpN 4.59 3.86 3.77 3.77 3.50 3.80-3.92
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4 | aDGlcp | 4.95 | 3.48 | 3.70 | 3.46 | 4.26 | 3.95
4.16 |
| 3,3,2 | Ac |
| 2.03 | |
| 3,3 | bDGlcpN | 4.65 | 3.62 | 3.58 | 3.44 | 3.44 | 3.77
3.94 |
| 3 | aDGalp | 5.46 | 3.96 | 3.85 | 4.28 | 3.94 | 3.76
3.81 |
| 2 | Ac |
| 2.08 | |
| | bDGlcpN | 4.59 | 3.86 | 3.77 | 3.77 | 3.50 | 3.80
3.92 |
|
There is only one chemically distinct structure:
Found 
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