Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 16784735Publication DOI: 10.1016/j.carres.2006.05.016Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: perepel
ioc.ac.ru
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 HongDa Street, TEDA, Tianjin, China, ianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, 23 HongDa Street, TEDA, Tianjin, China
teichoic acid-like O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide (LPS) of Escherichia coli O29. The O-polysaccharide and an oligosaccharide obtained by dephosphorylation of the O-polysaccharide were studied by sugar analysis along with 1H and 13C NMR spectroscopy. The following structure of the branched oligosaccharide repeating unit, containing five monosaccharide residues and glycerol 1-phosphate (D-Gro-1-P), was established: [carbohydrate structure: see text].
NMR, O-antigen, Escherichia coli, O-polysaccharide, bacterial polysaccharide structure, glycerol phosphate
Structure type: oligomer
Location inside paper: p.2178
Trivial name: O-unit of polysaccharide
Contained glycoepitopes: IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_983931,SB_192,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, 31P NMR, GLC
Related record ID(s): 20208
NCBI Taxonomy refs (TaxIDs): 1095711Reference(s) to other database(s): GTC:G32573EA
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,3,6 aDGlcp 98.3 72.5 75.8 71.0 73.0 62.0
3,3,3 aDGalp 100.8 70.0 71.0 71.0 71.0 67.0
3,3,2 Ac 175.5 23.4-23.7
3,3,4 bDGlcp 104.3 75.2 77.1 71.0 77.3 62.4
3,3 aLFucpN 99.5 50.5 72.2 79.1 69.4 16.9
3,2 Ac 175.5 23.4-23.7
3 bDGlcpN 103.0 56.9 80.0 70.1 77.6 62.3
xDGro 63.9 71.9 72.3
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,3,6 aDGlcp 4.92 3.58 3.55 3.45 3.80 3.76-3.87
3,3,3 aDGalp 5.11 3.89 3.89 4.01 4.35 3.56-3.89
3,3,2 Ac - 1.98-2.02
3,3,4 bDGlcp 4.63 3.42 3.51 3.45 3.48 3.76-3.87
3,3 aLFucpN 5.00 4.45 4.06 4.24 4.50 1.31
3,2 Ac - 1.98-2.02
3 bDGlcpN 4.46 3.87 3.69 3.65 3.43 3.67-3.94
xDGro 3.51-3.60 3.83 3.60-3.93
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,3,6 aDGlcp 98.3/4.92 72.5/3.58 75.8/3.55 71.0/3.45 73.0/3.80 62.0/3.76-3.87
3,3,3 aDGalp 100.8/5.11 70.0/3.89 71.0/3.89 71.0/4.01 71.0/4.35 67.0/3.56-3.89
3,3,2 Ac 23.4-23.7/1.98-2.02
3,3,4 bDGlcp 104.3/4.63 75.2/3.42 77.1/3.51 71.0/3.45 77.3/3.48 62.4/3.76-3.87
3,3 aLFucpN 99.5/5.00 50.5/4.45 72.2/4.06 79.1/4.24 69.4/4.50 16.9/1.31
3,2 Ac 23.4-23.7/1.98-2.02
3 bDGlcpN 103.0/4.46 56.9/3.87 80.0/3.69 70.1/3.65 77.6/3.43 62.3/3.67-3.94
xDGro 63.9/3.51-3.60 71.9/3.83 72.3/3.60-3.93
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,3,6 | aDGlcp | 4.92 | 3.58 | 3.55 | 3.45 | 3.80 | 3.76
3.87 |
| 3,3,3 | aDGalp | 5.11 | 3.89 | 3.89 | 4.01 | 4.35 | 3.56
3.89 |
| 3,3,2 | Ac |
| 1.98
2.02 | |
| 3,3,4 | bDGlcp | 4.63 | 3.42 | 3.51 | 3.45 | 3.48 | 3.76
3.87 |
| 3,3 | aLFucpN | 5.00 | 4.45 | 4.06 | 4.24 | 4.50 | 1.31 |
| 3,2 | Ac |
| 1.98
2.02 | |
| 3 | bDGlcpN | 4.46 | 3.87 | 3.69 | 3.65 | 3.43 | 3.67
3.94 |
| | xDGro | 3.51
3.60 | 3.83 | 3.60
3.93 | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,3,6 | aDGlcp | 98.3 | 72.5 | 75.8 | 71.0 | 73.0 | 62.0 |
| 3,3,3 | aDGalp | 100.8 | 70.0 | 71.0 | 71.0 | 71.0 | 67.0 |
| 3,3,2 | Ac | 175.5 | 23.4
23.7 | |
| 3,3,4 | bDGlcp | 104.3 | 75.2 | 77.1 | 71.0 | 77.3 | 62.4 |
| 3,3 | aLFucpN | 99.5 | 50.5 | 72.2 | 79.1 | 69.4 | 16.9 |
| 3,2 | Ac | 175.5 | 23.4
23.7 | |
| 3 | bDGlcpN | 103.0 | 56.9 | 80.0 | 70.1 | 77.6 | 62.3 |
| | xDGro | 63.9 | 71.9 | 72.3 | |
|
There is only one chemically distinct structure:
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Found 1 record.
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