Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Acinetobacter baumannii [ICD11:
XN8LS 
]
The structure was elucidated in this paperNCBI PubMed ID: 37975684Publication DOI: 10.1128/spectrum.03025-23Journal NLM ID: 101634614Publisher: Washington, DC: ASM Press
Correspondence: J.J. Kenyon <j.kenyon
griffith.edu.au>
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia, M. M. Shemyakin and Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia, Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia, School of Life and Environmental Sciences, Faculty of Science, University of Sydney, Sydney, Australia, Saw Swee Hock School of Public Health, National University of Singapore, Queenstown, Singapore, Yong Loo Lin School of Medicine, National University of Singapore, Queenstown, Singapore
Bacteriophage show promise for the treatment of Acinetobacter baumannii infections that resist all therapeutically suitable antibiotics. Many tail-spike depolymerases encoded by phage that are able to degrade A. baumannii capsular polysaccharide (CPS) exhibit specificity for the linkage present between K-units that make up CPS polymers. This linkage is formed by a specific Wzy polymerase, and the ability to predict this linkage using sequence-based methods that identify the Wzy at the K locus could assist with the selection of phage for therapy. However, little is known about the specificity of Wzy polymerase enzymes. Here, we describe a Wzy polymerase that can accommodate two different but similar sugars as one of the residues it links and phage depolymerases that can cleave both types of bond that Wzy forms.
Acinetobacter baumannii, capsular polysaccharide, Wzy polymerase, phage depolymerase, K70
Structure type: polymer chemical repeating unit
Location inside paper: table 2, Fig. 3A, K70 CPS
Compound class: CPS
Methods: 13C NMR, 1H NMR, NMR-2D, DNA sequencing, sugar analysis, DNA techniques, GLC, Smith degradation, HPLC, GPC, bioinformatic analysis, phage depolymerisation, HR-ESI-MS
Enzymes that release or process the structure: Gtr21,Gtr22, Gtr31, Wzy(kL9)[ItrB3]
Comments, role: KL type: KL70
Related record ID(s): 20742, 20743, 20744, 20745, 20746, 20747, 20748, 20749, 20750, 20751, 20752, 20753, 20754, 20755, 22258
NCBI Taxonomy refs (TaxIDs): 470
Show glycosyltransferases
NMR conditions: in D2O at 333 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,2 Ac 174.1-174.8 23.6-23.8
3,3,4,2 Ac 174.1-174.8 23.6-23.8
3,3,4 aLFucpN 97.9 50.8 68.9 72.4 68.3 16.5
3,3 aDGalpNA 99.8 49.8 76.8 75.2 72.4 175.7
3,2 Ac 174.1-174.8 23.6-23.8
3 aLFucpN 100.1 49.8 74.6 72.3 68.2 16.5
2 Ac 174.1-174.8 23.6-23.8
bDFucpN 102.9 53.2 77.5 68.3 72.4 16.8
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,2 Ac - 1.91-2.03
3,3,4,2 Ac - 1.91-2.03
3,3,4 aLFucpN 5.48 4.09 3.91 3.76 4.09 1.19
3,3 aDGalpNA 5.13 4.45 4.20 4.63 4.62 -
3,2 Ac - 1.91-2.03
3 aLFucpN 4.88 4.30 4.10 3.68 3.99 1.28
2 Ac - 1.91-2.03
bDFucpN 4.59 3.78 3.91 3.99 3.76 1.19
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,2 Ac 23.6-23.8/1.91-2.03
3,3,4,2 Ac 23.6-23.8/1.91-2.03
3,3,4 aLFucpN 97.9/5.48 50.8/4.09 68.9/3.91 72.4/3.76 68.3/4.09 16.5/1.19
3,3 aDGalpNA 99.8/5.13 49.8/4.45 76.8/4.20 75.2/4.63 72.4/4.62
3,2 Ac 23.6-23.8/1.91-2.03
3 aLFucpN 100.1/4.88 49.8/4.30 74.6/4.10 72.3/3.68 68.2/3.99 16.5/1.28
2 Ac 23.6-23.8/1.91-2.03
bDFucpN 102.9/4.59 53.2/3.78 77.5/3.91 68.3/3.99 72.4/3.76 16.8/1.19
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,2 | Ac |
| 1.91
2.03 | |
| 3,3,4,2 | Ac |
| 1.91
2.03 | |
| 3,3,4 | aLFucpN | 5.48 | 4.09 | 3.91 | 3.76 | 4.09 | 1.19 |
| 3,3 | aDGalpNA | 5.13 | 4.45 | 4.20 | 4.63 | 4.62 |
|
| 3,2 | Ac |
| 1.91
2.03 | |
| 3 | aLFucpN | 4.88 | 4.30 | 4.10 | 3.68 | 3.99 | 1.28 |
| 2 | Ac |
| 1.91
2.03 | |
| | bDFucpN | 4.59 | 3.78 | 3.91 | 3.99 | 3.76 | 1.19 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,2 | Ac | 174.1
174.8 | 23.6
23.8 | |
| 3,3,4,2 | Ac | 174.1
174.8 | 23.6
23.8 | |
| 3,3,4 | aLFucpN | 97.9 | 50.8 | 68.9 | 72.4 | 68.3 | 16.5 |
| 3,3 | aDGalpNA | 99.8 | 49.8 | 76.8 | 75.2 | 72.4 | 175.7 |
| 3,2 | Ac | 174.1
174.8 | 23.6
23.8 | |
| 3 | aLFucpN | 100.1 | 49.8 | 74.6 | 72.3 | 68.2 | 16.5 |
| 2 | Ac | 174.1
174.8 | 23.6
23.8 | |
| | bDFucpN | 102.9 | 53.2 | 77.5 | 68.3 | 72.4 | 16.8 |
|
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
