Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: cystic fibrosis (CF) [ICD11:
CA25 
];
infection due to Pseudomonas aeruginosa [ICD11:
XN5L6 ]
The structure was elucidated in this paperNCBI PubMed ID: 35653593Publication DOI: 10.1021/acsinfecdis.2c00183Journal NLM ID: 101654580Publisher: Washington, DC: American Chemical Society
Correspondence: A.D. Cox <Andrew.Cox
nrc-cnrc.gc.ca>
Institutions: Vaccine and Emerging Infections Research, Human Health Therapeutics Research Centre, National Research Council, Ottawa, Ontario K1A 0R6, Canada
Pseudomonas aeruginosa produces a variety of cell surface glycans. Previous studies identified a common polysaccharide (PS) antigen often termed A-band PS that was composed of a neutral d-rhamnan trisaccharide repeating unit as a relatively conserved cell surface carbohydrate. However, nuclear magnetic resonance (NMR) spectra and chemical analysis of A-PS preparations showed the presence of several additional components. Here, we report the characterization of the carbohydrate component responsible for these signals. The carbohydrate antigen consists of an immunogenic methylated rhamnan oligosaccharide at the nonreducing end of the A-band PS. Initial studies performed with the isolated antigen permitted the production of conjugates that were used to immunize mice and rabbits and generate monoclonal and polyclonal antibodies. The polyclonal antibodies were able to recognize the majority of P. aeruginosa strains in our collection, and three monoclonal antibodies were generated, one of which was able to recognize and facilitate opsonophagocytic killing of a majority of P. aeruginosa strains. This monoclonal antibody was able to recognize all P. aeruginosa strains in our collection that includes clinical and serotype strains. Synthetic oligosaccharides (mono- to pentasaccharides) representing the terminal 3-O-methyl d-rhamnan were prepared, and the trisaccharide was identified as the antigenic determinant required to effectively mimic the natural antigen recognized by the broadly cross-reactive monoclonal antibody. These data suggest that there is considerable promise in this antigen as a vaccine or therapeutic target.
Lipopolysaccharide, Pseudomonas aeruginosa, monoclonal antibody, glycoconjugate vaccine, common polysaccharide antigen, methylrhamnan
Structure type: oligomer
Location inside paper: p. 1337, Fig. 1, OS2
Aglycon: BSA, CRM197
Trivial name: A-band polysaccharide (A-PS)
Compound class: CPA
Contained glycoepitopes: IEDB_130701,IEDB_1394181,IEDB_144983,IEDB_152206,IEDB_983930,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, periodate oxidation, NMR-2D, GC-MS, ELISA, ESI-MS, anion-exchange chromatography, MALDI-MS, chemical synthesis, HPLC, serum bactericidal assays, gel chromatography, SEC, SPR, immunization, conjugation, alkaline treatment, opsonophagocytic assay, isolation, mAb epitope mapping
Comments, role: Double periodate oxidized A-PS for conjugation led to oligosaccharide OS2 with 3-deuterated glyceraldehyde at the reducing end.
Related record ID(s): 8437, 20856, 20857, 20859, 20860
NCBI Taxonomy refs (TaxIDs): 208964
Show glycosyltransferases
There is only one chemically distinct structure:
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