Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Acinetobacter baumannii [ICD11:
XN8LS 
]
The structure was elucidated in this paperNCBI PubMed ID: 10520267Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: S.G.Wilkison
chem.hull.ac.uk
Institutions: Department of Chemistry, University of Hull, UK
A polymeric fraction containing D-galactose, N-acetyl-D-galactosamine, and N-acetyl-D-glucosamine was isolated from the lipopolysaccharide produced by the reference strain for Acinetobacter baumannii serogroup O1. By means of NMR spectroscopy, methylation analysis, and chemical degradation, the repeating unit of the polymer was identified as a branched trisaccharide of the following structure. [formula: see text]
structure, polysaccharide, Acinetobacter, Acinetobacter baumannii, O-specific, O-specific polysaccharide, serogroup
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.207, structure 3
Compound class: CPS, O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_141807,IEDB_151528,IEDB_151531,IEDB_190606,SB_7
Methods: methylation, NMR-2D, NMR, sugar analysis
Related record ID(s): 2196
NCBI Taxonomy refs (TaxIDs): 470Reference(s) to other database(s): GTC:G70774JU, GlycomeDB:
25556
Show glycosyltransferases
NMR conditions: in D2O at 343 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,2 Ac
3,6 aDGalp 99.54 69.70 70.78 70.48 72.24 62.22
3 aDGlcpN 94.42 53.02 81.26 72.07 69.59 66.77
2 Ac
bDGalpN 101.99 52.23 75.78 64.54 76.10 62.01
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,2 Ac
3,6 aDGalp 5.00 3.86 3.89 4.03 3.74 3.76
3 aDGlcpN 5.00 4.10 3.75 3.96 3.74 3.75-4.03
2 Ac
bDGalpN 4.60 4.04 3.83 4.11 3.67 3.80
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,2 Ac
3,6 aDGalp 99.54/5.00 69.70/3.86 70.78/3.89 70.48/4.03 72.24/3.74 62.22/3.76
3 aDGlcpN 94.42/5.00 53.02/4.10 81.26/3.75 72.07/3.96 69.59/3.74 66.77/3.75-4.03
2 Ac
bDGalpN 101.99/4.60 52.23/4.04 75.78/3.83 64.54/4.11 76.10/3.67 62.01/3.80
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,2 | Ac | |
| 3,6 | aDGalp | 5.00 | 3.86 | 3.89 | 4.03 | 3.74 | 3.76 |
| 3 | aDGlcpN | 5.00 | 4.10 | 3.75 | 3.96 | 3.74 | 3.75
4.03 |
| 2 | Ac | |
| | bDGalpN | 4.60 | 4.04 | 3.83 | 4.11 | 3.67 | 3.80 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,2 | Ac | |
| 3,6 | aDGalp | 99.54 | 69.70 | 70.78 | 70.48 | 72.24 | 62.22 |
| 3 | aDGlcpN | 94.42 | 53.02 | 81.26 | 72.07 | 69.59 | 66.77 |
| 2 | Ac | |
| | bDGalpN | 101.99 | 52.23 | 75.78 | 64.54 | 76.10 | 62.01 |
|
There is only one chemically distinct structure:
Found 
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