Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Shigella flexneri [ICD11:
XN7Y2 
]
The structure was elucidated in this paperNCBI PubMed ID: 35773292Publication DOI: 10.1038/s41541-022-00497-7Journal NLM ID: 101699863Publisher: London: Springer Nature, the Sealy Center for Vaccine Development
Correspondence: francesca.x.micoli
gsk.com
Institutions: GSK Vaccines Institute for Global Health (GVGH), Siena, Italy, Universita di Trieste, Trieste, Italy, University of Cape Town, Rondebosch, South Africa
Generalized modules for membrane antigens (GMMA) are exosomes released from engineered Gram-negative bacteria and represent an attractive vaccine platform for the delivery of the O-Antigen (OAg), recognized as the key target for protective immunity against several pathogens such as Shigella. Shigella is a major cause of disease in Low- and Middle-Income countries and the development of a vaccine needs to deal with its large serotypic diversity. All S. flexneri serotypes, except serotype 6, share a conserved OAg backbone, corresponding to serotype Y. Here, a GMMA-producing S. flexneri scaffold strain displaying the OAg backbone was engineered with different OAg-modifying enzymes, either individually or in combinations. This strategy rapidly yielded GMMA displaying 12 natural serotypes and 16 novel serotypes expressing multiple epitopes combinations that do not occur in nature. Importantly, a candidate GMMA displaying a hybrid OAg elicited broadly cross-bactericidal antibodies against a large panel of S. flexneri serotypes.
O-antigen, vaccines, GMMA, S.flexneri serotypes
Structure type: polymer chemical repeating unit
Location inside paper: Fig. S1, Fig. 1a, table S2, S. flexneri GMMA 4b scaffold
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_125613,IEDB_125614,IEDB_127514,IEDB_130422,IEDB_133752,IEDB_133753,IEDB_133754,IEDB_135813,IEDB_135849,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_141815,IEDB_141816,IEDB_142488,IEDB_143253,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_153213,IEDB_225177,IEDB_885823,IEDB_983931,SB_192
Methods: 1H NMR, GLC-MS, NMR-2D, PCR, HPAEC-PAD, immunization, HPLC-SEC, FACS assay, immunogenicity studies, genetic engineering, GMMA-technology
Enzymes that release or process the structure: gtrIV, oacA
Comments, role: OAg isolated from S. flexneri GMMA 4b scaffold (conversion of S. flexneri GMMA Y scaffold + gtrIV + oacA); Shigella flexneri scaffold ΔtolR::frt pCOLA-Duet_gtrIV pACYC-Duet_oacA.
Related record ID(s): 8464, 20961, 20962, 20963, 20964, 20965, 20966, 20967, 20968, 20969, 20970, 20971, 20972, 20974, 20975, 20976
NCBI Taxonomy refs (TaxIDs): 623Reference(s) to other database(s): GTC:G36735BW, GlycomeDB:
37319
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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