Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 17888872Journal NLM ID: 0372516Publisher: Academic Press
Correspondence: zhanghoucheng
sdu.edu.cn
Institutions: The State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong 250100, China
GDP-perosamine synthetase (Per, E.C. not yet classified) is important to the synthesis of Escherichia coli O157:H7 O-antigen. The mutant in per gene can disrupt the synthesis of O157 O-antigen. In this study, GDP-perosamine synthetase was cloned from E. coli O157:H7 and over-expressed in E. coli BL21 (DE3). The recombinant His-tagged Per fusion protein was a decamer with molecular weight of 431 kDa. The optimal pH value of this recombinant protein was 7.5. The divalent ions had no significant effect on Per-catalyzed reaction. The K(m) and K(cat)/K(m) for GDP-4-keto-6-deoxy-d-mannose were 0.09 mM and 2.1 x 10(5)M(-1)S(-1), and those for l-glutamate were 2mM and 0.52 x 10(5)M(-1)S(-1), respectively. Per was used to synthesize GDP-perosamine from GDP-mannose together with recombinant GDP-mannose dehydratase (GMD, E.C. 4.2.1.47). The purified GDP-perosamine was identified by MS and NMR. In summary, this work provided a feasible approach for the synthesis of GDP-perosamine which can lead to the study of LPS biosynthesis of pathogenic E. coli O157:H7
E. coli O157:H7, GDP-perosamine, GDP-perosamine synthetase, Per, Drug design
Structure type: polymer chemical repeating unit
Location inside paper: p.526, fig.1A
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_136045,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_146664,IEDB_152214,IEDB_174333,IEDB_885822,IEDB_983931,SB_192,SB_86
Methods: 13C NMR, 1H NMR, SDS-PAGE, ESI-MS, genetic methods, biochemical methods, capillary electrophoresis (CE)
Biosynthesis and genetic data: genetic data, biochemical data
Synthetic data: enzymatic
Related record ID(s): 8988, 20690, 21655, 21656
NCBI Taxonomy refs (TaxIDs): 83334Reference(s) to other database(s): GTC:G60704LX, GlycomeDB:
3513
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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