Found 3 publications. Displayed publications from 1 to 3
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1. (Article ID: 215)
 
Warnecke JM, Nitschke M, Moolenaar CEC, Rietschel ET, Hartmann RK, Mamat U
The 5'-proximal hairpin loop of lbi RNA is a key structural element in repression of D-galactan II biosynthesis in Klebsiella pneumoniae serotype O1
Molecular Microbiology 36(3) (2000) 697-709
 

The lbi (lipopolysaccharide biosynthesis interfering) RNA of phage Acm1, an untranslated RNA transcript of 97 nucleotides, previously shown to affect Opolysaccharide biosynthesis in various Escherichia coli strains, was found to downregulate the synthesis of the D-galactan II component of the O-specific polysaccharide in Klebsiella pneumoniae serotype O1. Enzymatic and Pb21 probing experiments revealed that lbi RNA consists of two consecutive stemloop structures, the 50-proximal hairpin loop of 15 nucleotides being particularly accessible to single strand-specific probes. Based on the assumption that the 50-proximal hairpin loop may be involved in an antisense interaction with cellular target RNAs, we randomly mutagenized one or two of its central nucleotides. Expression of mutated lbi RNA variants in K. pneumoniae serotype O1 relieved at least partly the repression of D-galactan II formation. In addition, a truncated version of lbi RNA lacking the 30-proximal hairpin loop was almost as efficient as the wild-type RNA in downregulating D-galactan II synthesis. The results obtained indicate that the 50-proximal hairpin loop of lbi RNA functions as a key structural element in the mechanism leading to the inhibition of D-galactan II biosynthesis in K. pneumoniae serotype O1.

biosynthesis, structural, serotype, Klebsiella, Klebsiella pneumoniae, D-galactan, repression, RNA

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2. (Article ID: 1778)
 
Knirel YA, Kochetkov NK
The structure of lipopolysaccharides of gram-negative bacteria. III. The structure of O-antigens: A review
Biochemistry (Moscow) 59(12) (1994) 1325-1383
 

This review summarizes data on the composition and structure of the O-antigens, the polysaccharide chains of the outer-membrane lipopolysaccharides (LPS) of Gram-negative bacteria defining the immunospecificity of these microbial cells. Special reference is given to some structural features of the O-antigens, such as the presence of unique monosaccharides and noncarbohydrate components, masked regularity, and the occurrence in one microorganism of LPS with structurally different polysaccharide chains. Antigenic relationships between microorganisms belonging to different taxonomic groups are discussed.

structure, O-antigen, chemical composition, bacterial lipopolysaccharides, Salmonella livingstone C1

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3. (Article ID: 2198)
 
Grimmecke HD, Mamat U, Voges M, Lauk W, Shashkov AS, Knirel YA
Structure of the extracellular polysaccharide of Acetobacter methanolicus MB 58/4 (IMET 10945)
Carbohydrate Research 218 (1991) 247-251
 

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