Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 17452091Journal NLM ID: 8709751Publisher: Academic Press
Correspondence: pina.fratamico
ars.usda.gov
Institutions: Eastern Regional Research Center, US Department of Agriculture, Agricultural Research Service, Wyndmoor, PA 19038, USA
The O-antigen gene clusters of Escherichia coli serogroups O117, O126, and O146 were sequenced, and 11, 10, and 11 open reading frames (ORFs) were identified, respectively. Genes required for O-antigen sugar biosynthesis, sugar transfer, and sugar processing were identified. Multiplex polymerase chain reaction (PCR) assays were developed targeting the wzx and wzy genes present in the O-antigen gene cluster of these serogroups. The assays were highly serogroup specific when tested against strains belonging to serogroups that were isolated from food, humans, animals, and environmental sources, as well as against representative strains belonging to ca. 165 different E. coli O serogroups and a number of non-E. coli bacteria. Thus, the results demonstrate that the wzx and wzy gene sequences were specific to E. coli O117, O126, and O146 and can be used as diagnostic markers for rapid identification and detection of these serogroups.
PCR, O-antigen, serogroup, serotyping, wzx, wzy, E. coli O117, E. coli O126, E. coli O146
Structure type: suggested polymer biological repeating unit
Location inside paper: p.298, fig.1a
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_134627,IEDB_136044,IEDB_136105,IEDB_137472,IEDB_137473,IEDB_1391961,IEDB_1391963,IEDB_141584,IEDB_141794,IEDB_142488,IEDB_143260,IEDB_144998,IEDB_146664,IEDB_190606,IEDB_225177,IEDB_885822,IEDB_885823,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_23,SB_24,SB_7,SB_8,SB_88
Methods: genetic methods
Biosynthesis and genetic data: genetic data
Comments, role: biological repeat frame was based on [PMID:19040662]
Related record ID(s): 3145, 20704, 24256, 28683
NCBI Taxonomy refs (TaxIDs): 2067430Reference(s) to other database(s): GTC:G65895LI, GlycomeDB:
28135
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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