Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 17888872Journal NLM ID: 0372516Publisher: Academic Press
Correspondence: zhanghoucheng
sdu.edu.cn
Institutions: The State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong 250100, China
GDP-perosamine synthetase (Per, E.C. not yet classified) is important to the synthesis of Escherichia coli O157:H7 O-antigen. The mutant in per gene can disrupt the synthesis of O157 O-antigen. In this study, GDP-perosamine synthetase was cloned from E. coli O157:H7 and over-expressed in E. coli BL21 (DE3). The recombinant His-tagged Per fusion protein was a decamer with molecular weight of 431 kDa. The optimal pH value of this recombinant protein was 7.5. The divalent ions had no significant effect on Per-catalyzed reaction. The K(m) and K(cat)/K(m) for GDP-4-keto-6-deoxy-d-mannose were 0.09 mM and 2.1 x 10(5)M(-1)S(-1), and those for l-glutamate were 2mM and 0.52 x 10(5)M(-1)S(-1), respectively. Per was used to synthesize GDP-perosamine from GDP-mannose together with recombinant GDP-mannose dehydratase (GMD, E.C. 4.2.1.47). The purified GDP-perosamine was identified by MS and NMR. In summary, this work provided a feasible approach for the synthesis of GDP-perosamine which can lead to the study of LPS biosynthesis of pathogenic E. coli O157:H7
E. coli O157:H7, GDP-perosamine, GDP-perosamine synthetase, Per, Drug design
Structure type: monomer
Location inside paper: p.526, fig.1B
Trivial name: GDP-4-keto-6-deoxy-D-mannose, GDP-4-keto-6-deoxymannose, GDP-6-deoxy-α-D-lyxo-hex-4-ulose, GDP-4-keto-6-deoxy-α-D-mannose, GDP-6-deoxy-D-lyxo-hexos-4-ulose
Compound class: nucleoside diphosphate sugar
Contained glycoepitopes: IEDB_141493,IEDB_149170,IEDB_190357
Methods: 13C NMR, 1H NMR, SDS-PAGE, ESI-MS, genetic methods, biochemical methods, capillary electrophoresis (CE)
Enzymes that release or process the structure: GDP-mannose dehydratase (GMD)
Biosynthesis and genetic data: genetic data, biochemical data
Synthetic data: enzymatic
Comments, role: intermediate of biosynthetic pathway GDP-perosamine
Related record ID(s): 21404, 21656, 22013, 22626, 22871, 22877, 22968, 23108, 23536, 23921, 24052
NCBI Taxonomy refs (TaxIDs): 83334
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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