Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 17600060Publication DOI: 10.1099/mic.0.2007/004192-0Journal NLM ID: 0376646Publisher: Washington, DC: Kluwer Academic/Plenum Publishers
Correspondence: wanglei
nankai.edu.cn
Institutions: TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 HongDa Street,TEDA, Tianjin 300457, P. R. China,Tianjin Key Laboratory for Microbial Functional Genomics, Nankai University, 23 HongDa Street, TEDA, Tianjin 300457, P. R. China, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, School of Molecular and Microbial Biosciences (G08), University of Sydney, Sydney, NSW 2006, Australia
The O-antigen moiety of the LPS is one of the most variable cell surface components of the Gram-negative bacterial outer membrane. Variation is due to the presence of different sugars and sugar linkages. Here, it is reported that a group of Escherichia coli O serogroups (O17, O44, O73, O77 and O106), and the Salmonella enterica serogroup O: 6,14 (H), share a common four-sugar backbone O-subunit structure, and possess almost identical O-antigen gene clusters. Whereas the E. coli O77 antigen does not have any substitutions, the other O antigens in this group differ by the addition of one or two glucose side branches at various positions of the backbone. The O-antigen gene clusters for all members of the group encode only the proteins required for biosynthesis of the common four-sugar backbone. The identification of three genes within a putative prophage in the E. coli O44 genome is also reported; these genes are presumably involved in the glucosylation of the basic tetrasaccharide unit. This was confirmed y deletion of one of the genes, which encodes a putative glucosyltransferase. Structural analysis of the O antigen produced by the mutant strain demonstrated the absence of glucosylation. An O-antigen structure shared by five E. coli and one S. enterica serogroups, all of which have a long evolutionary history, suggests that the common backbone may be important for the survival of E.coli strains in the environment, or for their pathogenicity.
Escherichia coli, TOCSY, ROESY, GlcNAc, N-acetyl-d-glucosamine, rotating Overhauser effect spectroscopy, total correlation spectroscopy
Structure type: suggested polymer biological repeating unit
Location inside paper: p.2160, fig.1, E. coli O17
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_137340,IEDB_137485,IEDB_140116,IEDB_141807,IEDB_141830,IEDB_142488,IEDB_143632,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_152206,IEDB_983930,IEDB_983931,SB_136,SB_192,SB_196,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, chemical analysis, GLC, genetic methods
Biosynthesis and genetic data: genetic data
Related record ID(s): 359, 3411, 20644, 21421, 21694, 21695, 23197, 30343
NCBI Taxonomy refs (TaxIDs): 1010800Reference(s) to other database(s): GTC:G77194DN, GlycomeDB:
37032
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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