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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11: XN6P4

]

NCBI PubMed ID: 12591884
Journal NLM ID: 2985120R
Publisher: American Society for Microbiology
Correspondence: cwhitfie

uoguelph.ca
Institutions: Department of Microbiology, University of Guelph, Guelph, Ontario N1G 2W1, Canada. Biophysics. Analytical Biochemistry, Research Center Borstel, Center for Medicine and Biosciences, D-23845 Borstel, Germany

The waa gene cluster is responsible for the biosynthesis of the lipopolysaccharide (LPS) core region in Escherichia coli and Salmonella: Homologs of the waaZ gene product are encoded by the waa gene clusters of Salmonella enterica and E. coli strains with the K-12 and R2 core types. Overexpression of WaaZ in E. coli and S. enterica led to a modified LPS structure showing core truncations and (where relevant) to a reduction in the amount of O-polysaccharide side chains. Mass spectrometry and nuclear magnetic resonance spectroscopy were used to determine the predominant LPS structures in an E. coli isolate with an R1 core (waaZ is lacking from the type R1 waa gene cluster) with a copy of the waaZ gene added on a plasmid. Novel truncated LPS structures, lacking up to 3 hexoses from the outer core, resulted from WaaZ overexpression. The truncated molecules also contained a KdoIII residue not normally found in the R1 core

Lipopolysaccharide, biosynthesis, LPS, structure, core, gene, isolate, microbiology, strain, polysaccharide, cell, chain, molecule, Research, side chain, Escherichia, Escherichia coli, type, predominant, cluster, gene cluster, O-polysaccharide, O polysaccharide, spectrometry, Salmonella, core region, region, mass spectrometry, reduction, biochemistry, biophysics, hexose, Hexoses, homolog, inner core, lead, Magnetic Resonance Spectroscopy, medicine, modification, modified, nuclear, nuclear magnetic resonance, nuclear magnetic resonance spectroscopy, plasmid, resonance, Salmonella enterica, spectroscopy, surface

Structure type: oligomer
Location inside paper: Fig. 5A
Aglycon: lipid A
Trivial name: core oligosaccharide
Compound class: LPS
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130659,IEDB_130670,IEDB_131186,IEDB_133751,IEDB_135818,IEDB_136906,IEDB_137472,IEDB_140088,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_153543,IEDB_190606,IEDB_2189047,IEDB_226811,IEDB_983931,SB_192,SB_7

Biosynthesis and genetic data: genetic data, biosynthetic data

Related record ID(s): 2079, 2188, 2189
NCBI Taxonomy refs (TaxIDs): 562
Reference(s) to other database(s): GTC:G25429ZB
Show glycosyltransferases

There is only one chemically distinct structure:

NCCOP(=O)(O)O[C@H](CO)[C@H]1O[C@@](O[C@@H]2C[C@](O)(C(=O)O)O[C@H]([C@H](O)CO)[C@@H]2O[C@H]2O[C@H]([C@@H](O)CO)[C@@H](OP(=O)(O)O)[C@H](O[C@H]3O[C@H]([C@@H](O)CO[C@H]4O[C@H]([C@@H](O)CO[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5N)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H](OP(=O)(O)OP(=O)(O)OCCN)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O[C@@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@H]6O)[C@H]5O[C@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O[C@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)[C@H]4O)[C@@H]3O)[C@@H]2O)(C(=O)O)C[C@@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize