Show legend Show as text

Structure type: monomer ; 243.08558
C₉H₁₃O₅N₃
Trivial name: cytidine
Compound class: nucleoside
Contained glycoepitopes: IEDB_141494

• Article ID: 8967
  Tienaho J, Karonen M, Muilu-Mäkelä R, Wähälä K, Leon Denegri E, Franzén R, Karp M, Santala V, Sarjala T "Metabolic profiling of water-soluble compounds from the extracts of dark septate endophytic fungi (DSE) isolated from Scots pine (Pinus sylvestris L.) seedlings using UPLC–Orbitrap–MS" - Molecules 24(12) (2019) ID 2330
  

  Endophytes are microorganisms living inside plant hosts and are known to be beneficial for the host plant vitality. In this study, we isolated three endophytic fungus species from the roots of Scots pine seedlings growing on Finnish drained peatland setting. The isolated fungi belonged to dark septate endophytes (DSE). The metabolic profiles of the hot water extracts of the fungi were investigated using Ultrahigh Performance Liquid Chromatography with Diode Array Detection and Electron Spray Ionization source Mass Spectrometry with Orbitrap analyzer (UPLC-DAD-ESI-MS-Orbitrap). Out of 318 metabolites, we were able to identify 220, of which a majority was amino acids and peptides. Additionally, opine amino acids, amino acid quinones, Amadori compounds, cholines, nucleobases, nucleosides, nucleotides, siderophores, sugars, sugar alcohols and disaccharides were found, as well as other previously reported metabolites from plants or endophytes. Some differences of the metabolic profiles, regarding the amount and identity of the found metabolites, were observed even though the fungi were isolated from the same host. Many of the discovered metabolites have been described possessing biological activities and properties, which may make a favorable contribution to the host plant nutrient availability or abiotic and biotic stress tolerance.

  peptides, endophytes, metabolites, endophytic fungi, Phialocephala fortinii, Acephala applanata, Coniochaeta mutabilis, Humicolopsis cephalosporioides, Scots pine, UPLC–MS

  NCBI PubMed ID: 31242564
  Publication DOI: 10.3390/molecules24122330
  Journal NLM ID: 100964009
  Publisher: Basel, Switzerland: MDPI
  Correspondence: Karp M ; Santala V ; Muilu-Mäkelä R ; Sarjala T ; Karonen M ; Wähälä K ; Leon Denegri E ; Franzén R ; Tienaho J ; Tienaho J
  Institutions: Faculty of Natural Sciences and Engineering, Tampere University, Tampere, Finland, Natural Resources Institute Finland (Luke), Helsinki, Finland, Natural Chemistry Research Group, Department of Chemistry, University of Turku, Turku, Finland, Department of Chemistry, University of Helsinki, Helsinki, Finland, School of Chemical Engineering, Department of Chemistry and Materials Science, Aalto University, Espoo, Finland
  Methods: PCR, DNA techniques, extraction, cell growth, centrifugation, Orbitrap MS, BLAST, UHPLC-ESI-HRMS, UHPLC-DAD
  
   
  
  Acephala applanata A, Phialocephala fortinii R, Humicolopsis cephalosporioides S16, Coniochaeta mutabilis S16
  CSDB ID 49932 (all data & tools)

Show legend Show as text

Structure type: monomer ; 254.08980
C₁₁H₁₄O₅N₂
Compound class: nucleoside
Contained glycoepitopes: IEDB_149136

• Article ID: 8967
  Tienaho J, Karonen M, Muilu-Mäkelä R, Wähälä K, Leon Denegri E, Franzén R, Karp M, Santala V, Sarjala T "Metabolic profiling of water-soluble compounds from the extracts of dark septate endophytic fungi (DSE) isolated from Scots pine (Pinus sylvestris L.) seedlings using UPLC–Orbitrap–MS" - Molecules 24(12) (2019) ID 2330
  

  Endophytes are microorganisms living inside plant hosts and are known to be beneficial for the host plant vitality. In this study, we isolated three endophytic fungus species from the roots of Scots pine seedlings growing on Finnish drained peatland setting. The isolated fungi belonged to dark septate endophytes (DSE). The metabolic profiles of the hot water extracts of the fungi were investigated using Ultrahigh Performance Liquid Chromatography with Diode Array Detection and Electron Spray Ionization source Mass Spectrometry with Orbitrap analyzer (UPLC-DAD-ESI-MS-Orbitrap). Out of 318 metabolites, we were able to identify 220, of which a majority was amino acids and peptides. Additionally, opine amino acids, amino acid quinones, Amadori compounds, cholines, nucleobases, nucleosides, nucleotides, siderophores, sugars, sugar alcohols and disaccharides were found, as well as other previously reported metabolites from plants or endophytes. Some differences of the metabolic profiles, regarding the amount and identity of the found metabolites, were observed even though the fungi were isolated from the same host. Many of the discovered metabolites have been described possessing biological activities and properties, which may make a favorable contribution to the host plant nutrient availability or abiotic and biotic stress tolerance.

  peptides, endophytes, metabolites, endophytic fungi, Phialocephala fortinii, Acephala applanata, Coniochaeta mutabilis, Humicolopsis cephalosporioides, Scots pine, UPLC–MS

  NCBI PubMed ID: 31242564
  Publication DOI: 10.3390/molecules24122330
  Journal NLM ID: 100964009
  Publisher: Basel, Switzerland: MDPI
  Correspondence: Karp M ; Santala V ; Muilu-Mäkelä R ; Sarjala T ; Karonen M ; Wähälä K ; Leon Denegri E ; Franzén R ; Tienaho J ; Tienaho J
  Institutions: Faculty of Natural Sciences and Engineering, Tampere University, Tampere, Finland, Natural Resources Institute Finland (Luke), Helsinki, Finland, Natural Chemistry Research Group, Department of Chemistry, University of Turku, Turku, Finland, Department of Chemistry, University of Helsinki, Helsinki, Finland, School of Chemical Engineering, Department of Chemistry and Materials Science, Aalto University, Espoo, Finland
  Methods: PCR, DNA techniques, extraction, cell growth, centrifugation, Orbitrap MS, BLAST, UHPLC-ESI-HRMS, UHPLC-DAD
  
   
  
  Acephala applanata A, Phialocephala fortinii R, Humicolopsis cephalosporioides S16, Coniochaeta mutabilis S16
  CSDB ID 49933 (all data & tools)

Show legend Show as text

Structure type: monomer ; 244.06948
C₉H₁₂O₆N₂
Trivial name: pseudouridine
Compound class: nucleoside
Contained glycoepitopes: IEDB_149136

• Article ID: 8967
  Tienaho J, Karonen M, Muilu-Mäkelä R, Wähälä K, Leon Denegri E, Franzén R, Karp M, Santala V, Sarjala T "Metabolic profiling of water-soluble compounds from the extracts of dark septate endophytic fungi (DSE) isolated from Scots pine (Pinus sylvestris L.) seedlings using UPLC–Orbitrap–MS" - Molecules 24(12) (2019) ID 2330
  

  Endophytes are microorganisms living inside plant hosts and are known to be beneficial for the host plant vitality. In this study, we isolated three endophytic fungus species from the roots of Scots pine seedlings growing on Finnish drained peatland setting. The isolated fungi belonged to dark septate endophytes (DSE). The metabolic profiles of the hot water extracts of the fungi were investigated using Ultrahigh Performance Liquid Chromatography with Diode Array Detection and Electron Spray Ionization source Mass Spectrometry with Orbitrap analyzer (UPLC-DAD-ESI-MS-Orbitrap). Out of 318 metabolites, we were able to identify 220, of which a majority was amino acids and peptides. Additionally, opine amino acids, amino acid quinones, Amadori compounds, cholines, nucleobases, nucleosides, nucleotides, siderophores, sugars, sugar alcohols and disaccharides were found, as well as other previously reported metabolites from plants or endophytes. Some differences of the metabolic profiles, regarding the amount and identity of the found metabolites, were observed even though the fungi were isolated from the same host. Many of the discovered metabolites have been described possessing biological activities and properties, which may make a favorable contribution to the host plant nutrient availability or abiotic and biotic stress tolerance.

  peptides, endophytes, metabolites, endophytic fungi, Phialocephala fortinii, Acephala applanata, Coniochaeta mutabilis, Humicolopsis cephalosporioides, Scots pine, UPLC–MS

  NCBI PubMed ID: 31242564
  Publication DOI: 10.3390/molecules24122330
  Journal NLM ID: 100964009
  Publisher: Basel, Switzerland: MDPI
  Correspondence: Karp M ; Santala V ; Muilu-Mäkelä R ; Sarjala T ; Karonen M ; Wähälä K ; Leon Denegri E ; Franzén R ; Tienaho J ; Tienaho J
  Institutions: Faculty of Natural Sciences and Engineering, Tampere University, Tampere, Finland, Natural Resources Institute Finland (Luke), Helsinki, Finland, Natural Chemistry Research Group, Department of Chemistry, University of Turku, Turku, Finland, Department of Chemistry, University of Helsinki, Helsinki, Finland, School of Chemical Engineering, Department of Chemistry and Materials Science, Aalto University, Espoo, Finland
  Methods: PCR, DNA techniques, extraction, cell growth, centrifugation, Orbitrap MS, BLAST, UHPLC-ESI-HRMS, UHPLC-DAD
  
   
  
  Acephala applanata A, Phialocephala fortinii R, Humicolopsis cephalosporioides S16, Coniochaeta mutabilis S16
  CSDB ID 49934 (all data & tools)

Show legend Show as text

Structure type: monomer ; 244.06915
C₉H₁₂O₆N₂
Trivial name: uridine
Compound class: nucleoside

• Article ID: 8967
  Tienaho J, Karonen M, Muilu-Mäkelä R, Wähälä K, Leon Denegri E, Franzén R, Karp M, Santala V, Sarjala T "Metabolic profiling of water-soluble compounds from the extracts of dark septate endophytic fungi (DSE) isolated from Scots pine (Pinus sylvestris L.) seedlings using UPLC–Orbitrap–MS" - Molecules 24(12) (2019) ID 2330
  

  Endophytes are microorganisms living inside plant hosts and are known to be beneficial for the host plant vitality. In this study, we isolated three endophytic fungus species from the roots of Scots pine seedlings growing on Finnish drained peatland setting. The isolated fungi belonged to dark septate endophytes (DSE). The metabolic profiles of the hot water extracts of the fungi were investigated using Ultrahigh Performance Liquid Chromatography with Diode Array Detection and Electron Spray Ionization source Mass Spectrometry with Orbitrap analyzer (UPLC-DAD-ESI-MS-Orbitrap). Out of 318 metabolites, we were able to identify 220, of which a majority was amino acids and peptides. Additionally, opine amino acids, amino acid quinones, Amadori compounds, cholines, nucleobases, nucleosides, nucleotides, siderophores, sugars, sugar alcohols and disaccharides were found, as well as other previously reported metabolites from plants or endophytes. Some differences of the metabolic profiles, regarding the amount and identity of the found metabolites, were observed even though the fungi were isolated from the same host. Many of the discovered metabolites have been described possessing biological activities and properties, which may make a favorable contribution to the host plant nutrient availability or abiotic and biotic stress tolerance.

  peptides, endophytes, metabolites, endophytic fungi, Phialocephala fortinii, Acephala applanata, Coniochaeta mutabilis, Humicolopsis cephalosporioides, Scots pine, UPLC–MS

  NCBI PubMed ID: 31242564
  Publication DOI: 10.3390/molecules24122330
  Journal NLM ID: 100964009
  Publisher: Basel, Switzerland: MDPI
  Correspondence: Karp M ; Santala V ; Muilu-Mäkelä R ; Sarjala T ; Karonen M ; Wähälä K ; Leon Denegri E ; Franzén R ; Tienaho J ; Tienaho J
  Institutions: Faculty of Natural Sciences and Engineering, Tampere University, Tampere, Finland, Natural Resources Institute Finland (Luke), Helsinki, Finland, Natural Chemistry Research Group, Department of Chemistry, University of Turku, Turku, Finland, Department of Chemistry, University of Helsinki, Helsinki, Finland, School of Chemical Engineering, Department of Chemistry and Materials Science, Aalto University, Espoo, Finland
  Methods: PCR, DNA techniques, extraction, cell growth, centrifugation, Orbitrap MS, BLAST, UHPLC-ESI-HRMS, UHPLC-DAD
  
   
  
  Acephala applanata A, Phialocephala fortinii R, Humicolopsis cephalosporioides S16, Coniochaeta mutabilis S16
  CSDB ID 49935 (all data & tools)

Show legend Show as text

Structure type: monomer ; 267.09608
C₁₀H₁₃O₄N₅
Trivial name: adenosine
Compound class: nucleoside
Contained glycoepitopes: IEDB_140947

• Article ID: 8967
  Tienaho J, Karonen M, Muilu-Mäkelä R, Wähälä K, Leon Denegri E, Franzén R, Karp M, Santala V, Sarjala T "Metabolic profiling of water-soluble compounds from the extracts of dark septate endophytic fungi (DSE) isolated from Scots pine (Pinus sylvestris L.) seedlings using UPLC–Orbitrap–MS" - Molecules 24(12) (2019) ID 2330
  

  Endophytes are microorganisms living inside plant hosts and are known to be beneficial for the host plant vitality. In this study, we isolated three endophytic fungus species from the roots of Scots pine seedlings growing on Finnish drained peatland setting. The isolated fungi belonged to dark septate endophytes (DSE). The metabolic profiles of the hot water extracts of the fungi were investigated using Ultrahigh Performance Liquid Chromatography with Diode Array Detection and Electron Spray Ionization source Mass Spectrometry with Orbitrap analyzer (UPLC-DAD-ESI-MS-Orbitrap). Out of 318 metabolites, we were able to identify 220, of which a majority was amino acids and peptides. Additionally, opine amino acids, amino acid quinones, Amadori compounds, cholines, nucleobases, nucleosides, nucleotides, siderophores, sugars, sugar alcohols and disaccharides were found, as well as other previously reported metabolites from plants or endophytes. Some differences of the metabolic profiles, regarding the amount and identity of the found metabolites, were observed even though the fungi were isolated from the same host. Many of the discovered metabolites have been described possessing biological activities and properties, which may make a favorable contribution to the host plant nutrient availability or abiotic and biotic stress tolerance.

  peptides, endophytes, metabolites, endophytic fungi, Phialocephala fortinii, Acephala applanata, Coniochaeta mutabilis, Humicolopsis cephalosporioides, Scots pine, UPLC–MS

  NCBI PubMed ID: 31242564
  Publication DOI: 10.3390/molecules24122330
  Journal NLM ID: 100964009
  Publisher: Basel, Switzerland: MDPI
  Correspondence: Karp M ; Santala V ; Muilu-Mäkelä R ; Sarjala T ; Karonen M ; Wähälä K ; Leon Denegri E ; Franzén R ; Tienaho J ; Tienaho J
  Institutions: Faculty of Natural Sciences and Engineering, Tampere University, Tampere, Finland, Natural Resources Institute Finland (Luke), Helsinki, Finland, Natural Chemistry Research Group, Department of Chemistry, University of Turku, Turku, Finland, Department of Chemistry, University of Helsinki, Helsinki, Finland, School of Chemical Engineering, Department of Chemistry and Materials Science, Aalto University, Espoo, Finland
  Methods: PCR, DNA techniques, extraction, cell growth, centrifugation, Orbitrap MS, BLAST, UHPLC-ESI-HRMS, UHPLC-DAD
  
   
  
  Acephala applanata A, Phialocephala fortinii R, Humicolopsis cephalosporioides S16, Coniochaeta mutabilis S16
  CSDB ID 49936 (all data & tools)

Show legend Show as text

Structure type: monomer ; 267.09639
C₁₀H₁₃O₄N₅
Trivial name: 2'-deoxyguanosine
Compound class: nucleoside

• Article ID: 8967
  Tienaho J, Karonen M, Muilu-Mäkelä R, Wähälä K, Leon Denegri E, Franzén R, Karp M, Santala V, Sarjala T "Metabolic profiling of water-soluble compounds from the extracts of dark septate endophytic fungi (DSE) isolated from Scots pine (Pinus sylvestris L.) seedlings using UPLC–Orbitrap–MS" - Molecules 24(12) (2019) ID 2330
  

  Endophytes are microorganisms living inside plant hosts and are known to be beneficial for the host plant vitality. In this study, we isolated three endophytic fungus species from the roots of Scots pine seedlings growing on Finnish drained peatland setting. The isolated fungi belonged to dark septate endophytes (DSE). The metabolic profiles of the hot water extracts of the fungi were investigated using Ultrahigh Performance Liquid Chromatography with Diode Array Detection and Electron Spray Ionization source Mass Spectrometry with Orbitrap analyzer (UPLC-DAD-ESI-MS-Orbitrap). Out of 318 metabolites, we were able to identify 220, of which a majority was amino acids and peptides. Additionally, opine amino acids, amino acid quinones, Amadori compounds, cholines, nucleobases, nucleosides, nucleotides, siderophores, sugars, sugar alcohols and disaccharides were found, as well as other previously reported metabolites from plants or endophytes. Some differences of the metabolic profiles, regarding the amount and identity of the found metabolites, were observed even though the fungi were isolated from the same host. Many of the discovered metabolites have been described possessing biological activities and properties, which may make a favorable contribution to the host plant nutrient availability or abiotic and biotic stress tolerance.

  peptides, endophytes, metabolites, endophytic fungi, Phialocephala fortinii, Acephala applanata, Coniochaeta mutabilis, Humicolopsis cephalosporioides, Scots pine, UPLC–MS

  NCBI PubMed ID: 31242564
  Publication DOI: 10.3390/molecules24122330
  Journal NLM ID: 100964009
  Publisher: Basel, Switzerland: MDPI
  Correspondence: Karp M ; Santala V ; Muilu-Mäkelä R ; Sarjala T ; Karonen M ; Wähälä K ; Leon Denegri E ; Franzén R ; Tienaho J ; Tienaho J
  Institutions: Faculty of Natural Sciences and Engineering, Tampere University, Tampere, Finland, Natural Resources Institute Finland (Luke), Helsinki, Finland, Natural Chemistry Research Group, Department of Chemistry, University of Turku, Turku, Finland, Department of Chemistry, University of Helsinki, Helsinki, Finland, School of Chemical Engineering, Department of Chemistry and Materials Science, Aalto University, Espoo, Finland
  Methods: PCR, DNA techniques, extraction, cell growth, centrifugation, Orbitrap MS, BLAST, UHPLC-ESI-HRMS, UHPLC-DAD
  
   
  
  Acephala applanata A, Phialocephala fortinii R, Humicolopsis cephalosporioides S16, Coniochaeta mutabilis S16
  CSDB ID 49937 (all data & tools)

Show legend Show as text

Structure type: monomer ; 256.10554
C₁₁H₁₆O₅N₂
Trivial name: 3-methylthymidine
Compound class: nucleoside
Contained glycoepitopes: IEDB_227709

• Article ID: 8967
  Tienaho J, Karonen M, Muilu-Mäkelä R, Wähälä K, Leon Denegri E, Franzén R, Karp M, Santala V, Sarjala T "Metabolic profiling of water-soluble compounds from the extracts of dark septate endophytic fungi (DSE) isolated from Scots pine (Pinus sylvestris L.) seedlings using UPLC–Orbitrap–MS" - Molecules 24(12) (2019) ID 2330
  

  Endophytes are microorganisms living inside plant hosts and are known to be beneficial for the host plant vitality. In this study, we isolated three endophytic fungus species from the roots of Scots pine seedlings growing on Finnish drained peatland setting. The isolated fungi belonged to dark septate endophytes (DSE). The metabolic profiles of the hot water extracts of the fungi were investigated using Ultrahigh Performance Liquid Chromatography with Diode Array Detection and Electron Spray Ionization source Mass Spectrometry with Orbitrap analyzer (UPLC-DAD-ESI-MS-Orbitrap). Out of 318 metabolites, we were able to identify 220, of which a majority was amino acids and peptides. Additionally, opine amino acids, amino acid quinones, Amadori compounds, cholines, nucleobases, nucleosides, nucleotides, siderophores, sugars, sugar alcohols and disaccharides were found, as well as other previously reported metabolites from plants or endophytes. Some differences of the metabolic profiles, regarding the amount and identity of the found metabolites, were observed even though the fungi were isolated from the same host. Many of the discovered metabolites have been described possessing biological activities and properties, which may make a favorable contribution to the host plant nutrient availability or abiotic and biotic stress tolerance.

  peptides, endophytes, metabolites, endophytic fungi, Phialocephala fortinii, Acephala applanata, Coniochaeta mutabilis, Humicolopsis cephalosporioides, Scots pine, UPLC–MS

  NCBI PubMed ID: 31242564
  Publication DOI: 10.3390/molecules24122330
  Journal NLM ID: 100964009
  Publisher: Basel, Switzerland: MDPI
  Correspondence: Karp M ; Santala V ; Muilu-Mäkelä R ; Sarjala T ; Karonen M ; Wähälä K ; Leon Denegri E ; Franzén R ; Tienaho J ; Tienaho J
  Institutions: Faculty of Natural Sciences and Engineering, Tampere University, Tampere, Finland, Natural Resources Institute Finland (Luke), Helsinki, Finland, Natural Chemistry Research Group, Department of Chemistry, University of Turku, Turku, Finland, Department of Chemistry, University of Helsinki, Helsinki, Finland, School of Chemical Engineering, Department of Chemistry and Materials Science, Aalto University, Espoo, Finland
  Methods: PCR, DNA techniques, extraction, cell growth, centrifugation, Orbitrap MS, BLAST, UHPLC-ESI-HRMS, UHPLC-DAD
  
   
  
  Acephala applanata A, Phialocephala fortinii R, Humicolopsis cephalosporioides S16, Coniochaeta mutabilis S16
  CSDB ID 49938 (all data & tools)

Show legend Show as text

Structure type: monomer ; 556.15074
C₂₄H₂₈O₁₅
Trivial name: blumeoside A
Compound class: glycoside
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192

• Article ID: 8967
  Tienaho J, Karonen M, Muilu-Mäkelä R, Wähälä K, Leon Denegri E, Franzén R, Karp M, Santala V, Sarjala T "Metabolic profiling of water-soluble compounds from the extracts of dark septate endophytic fungi (DSE) isolated from Scots pine (Pinus sylvestris L.) seedlings using UPLC–Orbitrap–MS" - Molecules 24(12) (2019) ID 2330
  

  Endophytes are microorganisms living inside plant hosts and are known to be beneficial for the host plant vitality. In this study, we isolated three endophytic fungus species from the roots of Scots pine seedlings growing on Finnish drained peatland setting. The isolated fungi belonged to dark septate endophytes (DSE). The metabolic profiles of the hot water extracts of the fungi were investigated using Ultrahigh Performance Liquid Chromatography with Diode Array Detection and Electron Spray Ionization source Mass Spectrometry with Orbitrap analyzer (UPLC-DAD-ESI-MS-Orbitrap). Out of 318 metabolites, we were able to identify 220, of which a majority was amino acids and peptides. Additionally, opine amino acids, amino acid quinones, Amadori compounds, cholines, nucleobases, nucleosides, nucleotides, siderophores, sugars, sugar alcohols and disaccharides were found, as well as other previously reported metabolites from plants or endophytes. Some differences of the metabolic profiles, regarding the amount and identity of the found metabolites, were observed even though the fungi were isolated from the same host. Many of the discovered metabolites have been described possessing biological activities and properties, which may make a favorable contribution to the host plant nutrient availability or abiotic and biotic stress tolerance.

  peptides, endophytes, metabolites, endophytic fungi, Phialocephala fortinii, Acephala applanata, Coniochaeta mutabilis, Humicolopsis cephalosporioides, Scots pine, UPLC–MS

  NCBI PubMed ID: 31242564
  Publication DOI: 10.3390/molecules24122330
  Journal NLM ID: 100964009
  Publisher: Basel, Switzerland: MDPI
  Correspondence: Karp M ; Santala V ; Muilu-Mäkelä R ; Sarjala T ; Karonen M ; Wähälä K ; Leon Denegri E ; Franzén R ; Tienaho J ; Tienaho J
  Institutions: Faculty of Natural Sciences and Engineering, Tampere University, Tampere, Finland, Natural Resources Institute Finland (Luke), Helsinki, Finland, Natural Chemistry Research Group, Department of Chemistry, University of Turku, Turku, Finland, Department of Chemistry, University of Helsinki, Helsinki, Finland, School of Chemical Engineering, Department of Chemistry and Materials Science, Aalto University, Espoo, Finland
  Methods: PCR, DNA techniques, extraction, cell growth, centrifugation, Orbitrap MS, BLAST, UHPLC-ESI-HRMS, UHPLC-DAD
  
   
  
  Acephala applanata A, Phialocephala fortinii R, Humicolopsis cephalosporioides S16, Coniochaeta mutabilis S16
  CSDB ID 49939 (all data & tools)

Show legend Show as text

Structure type: monomer ; 226.09527
C₁₀H₁₄O₄N₂
Trivial name: 2'-deoxythymidine
Compound class: nucleoside
Contained glycoepitopes: IEDB_138113

• Article ID: 8967
  Tienaho J, Karonen M, Muilu-Mäkelä R, Wähälä K, Leon Denegri E, Franzén R, Karp M, Santala V, Sarjala T "Metabolic profiling of water-soluble compounds from the extracts of dark septate endophytic fungi (DSE) isolated from Scots pine (Pinus sylvestris L.) seedlings using UPLC–Orbitrap–MS" - Molecules 24(12) (2019) ID 2330
  

  Endophytes are microorganisms living inside plant hosts and are known to be beneficial for the host plant vitality. In this study, we isolated three endophytic fungus species from the roots of Scots pine seedlings growing on Finnish drained peatland setting. The isolated fungi belonged to dark septate endophytes (DSE). The metabolic profiles of the hot water extracts of the fungi were investigated using Ultrahigh Performance Liquid Chromatography with Diode Array Detection and Electron Spray Ionization source Mass Spectrometry with Orbitrap analyzer (UPLC-DAD-ESI-MS-Orbitrap). Out of 318 metabolites, we were able to identify 220, of which a majority was amino acids and peptides. Additionally, opine amino acids, amino acid quinones, Amadori compounds, cholines, nucleobases, nucleosides, nucleotides, siderophores, sugars, sugar alcohols and disaccharides were found, as well as other previously reported metabolites from plants or endophytes. Some differences of the metabolic profiles, regarding the amount and identity of the found metabolites, were observed even though the fungi were isolated from the same host. Many of the discovered metabolites have been described possessing biological activities and properties, which may make a favorable contribution to the host plant nutrient availability or abiotic and biotic stress tolerance.

  peptides, endophytes, metabolites, endophytic fungi, Phialocephala fortinii, Acephala applanata, Coniochaeta mutabilis, Humicolopsis cephalosporioides, Scots pine, UPLC–MS

  NCBI PubMed ID: 31242564
  Publication DOI: 10.3390/molecules24122330
  Journal NLM ID: 100964009
  Publisher: Basel, Switzerland: MDPI
  Correspondence: Karp M ; Santala V ; Muilu-Mäkelä R ; Sarjala T ; Karonen M ; Wähälä K ; Leon Denegri E ; Franzén R ; Tienaho J ; Tienaho J
  Institutions: Faculty of Natural Sciences and Engineering, Tampere University, Tampere, Finland, Natural Resources Institute Finland (Luke), Helsinki, Finland, Natural Chemistry Research Group, Department of Chemistry, University of Turku, Turku, Finland, Department of Chemistry, University of Helsinki, Helsinki, Finland, School of Chemical Engineering, Department of Chemistry and Materials Science, Aalto University, Espoo, Finland
  Methods: PCR, DNA techniques, extraction, cell growth, centrifugation, Orbitrap MS, BLAST, UHPLC-ESI-HRMS, UHPLC-DAD
  
   
  
  Acephala applanata A, Phialocephala fortinii R, Humicolopsis cephalosporioides S16, Coniochaeta mutabilis S16
  CSDB ID 49940 (all data & tools)