Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: cystic fibrosis (CF) [ICD11:
CA25 
]
NCBI PubMed ID: 37813217Publication DOI: 10.1016/j.ijbiomac.2023.127294Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: P. Cescutti <pcescutti
units.it>
Institutions: Department of Life Sciences, University of Trieste, Via Licio Giorgieri 1, 34127 Trieste, Italy, Department of Chemical and Pharmaceutical Sciences, INSTM UdR Trieste, University of Trieste, Via Licio Giorgieri 1, 34127 Trieste, Italy, Simpson Querrey Institute, Northwestern University, Chicago, IL 60611, USA, Department of Chemistry, Northwestern University, Evanston, IL 60208, USA, Food Science Department, Cornell University, 101A Stocking Hall, Ithaca, NY 14853, USA
Bacteria form very often biofilms where they embed in a self-synthesized matrix exhibiting a gel-like appearance. Matrices offer several advantages, including defence against external threats and the easiness of intercellular communication. In infections, biofilm formation enhances bacteria resistance against antimicrobials, causing serious clinical problems for patients' treatments. Biofilm matrices are composed of proteins, extracellular DNA, and polysaccharides, the latter being the major responsible for matrix architecture. The repeating unit of the biofilm polysaccharide synthesized by Burkholderia multivorans strain C1576 contains two mannoses and two sequentially linked rhamnoses, one of them 50 % methylated on C-3. Rhamnose, a 6-deoxysugar, has lower polarity than other common monosaccharides and its methylation further reduces polarity. This suggests a possible role of this polysaccharide in the biofilm matrix; in fact, computer modelling and atomic force microscopy studies evidenced intra- and inter-molecular non-polar interactions both within polysaccharides and with aliphatic molecules. In this paper, the polysaccharide three-dimensional morphology was investigated using atomic force microscopy in both solid and solution states. Independent evidence of the polymer conformation was obtained by transmission electron microscopy which confirmed the formation of globular compact structures. Finally, data from computer dynamic simulations were used to model the three-dimensional structure.
Polysaccharide functions, Burkholderia multivorans biofilm, polysaccharide AFM, TEM and modeling
Structure type: polymer chemical repeating unit
Location inside paper: Scheme 1, Epol C1576
Trivial name: rhamno-mannan, rhamnomannan, Epol C1576
Compound class: EPS
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_1394181,IEDB_143632,IEDB_144983,IEDB_145010,IEDB_152206,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: HPLC, HPSEC, TEM, AFM
3D data: molecular modeling, 3D model of polysaccharide was generated by the computer program CHARMM from both AFM and TEM images
NCBI Taxonomy refs (TaxIDs): 87883Reference(s) to other database(s): GTC:G72472ZR
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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