Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Clostridium difficile [ICD11:
XN0SE 
]
NCBI PubMed ID: 37965964Publication DOI: 10.1021/acsinfecdis.3c00485Journal NLM ID: 101654580Publisher: Washington, DC: American Chemical Society
Correspondence: P.J. Hensbergen <p.j.hensbergen
lumc.nl>
Institutions: Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom, Department of Medical Microbiology, Leiden University Medical Center, Leiden 2333 ZA, The Netherlands
The bacterial flagellum is involved in a variety of processes including motility, adherence, and immunomodulation. In the Clostridioides difficile strain 630Δerm, the main filamentous component, FliC, is post-translationally modified with an O-linked Type A glycan structure. This modification is essential for flagellar function, since motility is seriously impaired in gene mutants with improper biosynthesis of the Type A glycan. The cd0240-cd0244 gene cluster encodes the Type A biosynthetic proteins, but the role of each gene, and the corresponding enzymatic activity, have not been fully elucidated. Using quantitative mass spectrometry-based proteomics analyses, we determined the relative abundance of the observed glycan variations of the Type A structure in cd0241, cd0242, cd0243, and cd0244 mutant strains. Our data not only confirm the importance of CD0241, CD0242, and CD0243 but, in contrast to previous data, also show that CD0244 is essential for the biosynthesis of the Type A modification. Combined with additional bioinformatic analyses, we propose a revised model for Type A glycan biosynthesis.
biosynthesis, mass spectrometry, enzyme, glycosylation, flagella, Proteomics
Structure type: monomer
Location inside paper: Fig. 1(A), FliC type A glycan
Aglycon: Ser/Thr
Trivial name: FliC type A glycan
Contained glycoepitopes: IEDB_137340,IEDB_141807,IEDB_151531
Methods: LC-MS/MS, proteomics analysis, HPLC-MS/MS
NCBI Taxonomy refs (TaxIDs): 272563
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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(previously named: Clostridium difficile 630Δerm)