Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Cyprinus carpio
Associated disease: motile aeromonad septicemia (MAS)
The structure was elucidated in this paperNCBI PubMed ID: 37086562Publication DOI: 10.1016/j.carres.2023.108809Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: A. Turska-Szewczuk <anna.turska-szewczuk
mail.umcs.pl>
Institutions: Department of Genetics and Microbiology, Institute of Biological Sciences, M. Curie-Sklodowska University, Akademicka 19, 20-033, Lublin, Poland, Department of Omics Analyses, National Veterinary Research Institute, Partyzantow 57, 24-100, Pulawy, Poland, Department of Preclinical Sciences and Infectious Diseases, Faculty of Veterinary Medicine and Animal Science, Poznan University of Life Sciences, Wolynska 35, 60-637, Poznan, Poland
Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup. The lipopolysaccharide of A. sobria K928 was subjected to mild acid hydrolysis, and the O-specific polysaccharide, which was isolated by gel-permeation chromatography, was studied using sugar and methylation analyses and 1H and 13C NMR spectroscopy. The following structure of the branched O-specific polysaccharide repeating unit of A. sobria K928 was established. →2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases. The composition of the O-antigen genetic region was found to be consistent with the O-polysaccharide structure, and its organization was proposed.
O-antigen, NMR spectroscopy, O-antigen gene cluster, Aeromonas, Wzx/Wzy pathway
Structure type: polymer chemical repeating unit
Location inside paper: abstract, table 1, p. 108809-7, A. popoffii PGO1 K928
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136105,IEDB_225177,IEDB_885823
NCBI Taxonomy refs (TaxIDs): 646
Show glycosyltransferases
NMR conditions: in D2O at 305 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,4,3,3,3 lR3HOBut 175.2 46.1 66.3 23.1
3,4,3,3 aDFucp3N 96.5 67.1 52.2 71.8 68.1 16.3
3,4,3 aLRhap 102.5 75.8 76.1 72.1 70.6 18.0
3,4 bLRhap 101.5 71.7 81.9 72.2 73.2 18.0
3 aLRhap 103.4 71.0 70.2 83.7 68.7 18.0
2 Ac 175.4 23.6
bDFucpN 103.1 52.4 81.0 71.8 71.9 16.6
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,3,3,3 lR3HOBut - 2.51 4.24 1.27
3,4,3,3 aDFucp3N 5.15 4.03 4.41 3.79 4.49 1.21
3,4,3 aLRhap 5.19 4.35 4.01 3.42 3.91 1.30
3,4 bLRhap 4.75 4.16 3.69 3.50 3.50 1.35
3 aLRhap 4.85 3.91 3.91 3.59 3.89 1.29
2 Ac - 2.12
bDFucpN 4.89 4.01 3.56 3.78 3.72 1.26
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,4,3,3,3 lR3HOBut 46.1/2.51 66.3/4.24 23.1/1.27
3,4,3,3 aDFucp3N 96.5/5.15 67.1/4.03 52.2/4.41 71.8/3.79 68.1/4.49 16.3/1.21
3,4,3 aLRhap 102.5/5.19 75.8/4.35 76.1/4.01 72.1/3.42 70.6/3.91 18.0/1.30
3,4 bLRhap 101.5/4.75 71.7/4.16 81.9/3.69 72.2/3.50 73.2/3.50 18.0/1.35
3 aLRhap 103.4/4.85 71.0/3.91 70.2/3.91 83.7/3.59 68.7/3.89 18.0/1.29
2 Ac 23.6/2.12
bDFucpN 103.1/4.89 52.4/4.01 81.0/3.56 71.8/3.78 71.9/3.72 16.6/1.26
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,3,3,3 | lR3HOBut |
| 2.51 | 4.24 | 1.27 | |
| 3,4,3,3 | aDFucp3N | 5.15 | 4.03 | 4.41 | 3.79 | 4.49 | 1.21 |
| 3,4,3 | aLRhap | 5.19 | 4.35 | 4.01 | 3.42 | 3.91 | 1.30 |
| 3,4 | bLRhap | 4.75 | 4.16 | 3.69 | 3.50 | 3.50 | 1.35 |
| 3 | aLRhap | 4.85 | 3.91 | 3.91 | 3.59 | 3.89 | 1.29 |
| 2 | Ac |
| 2.12 | |
| | bDFucpN | 4.89 | 4.01 | 3.56 | 3.78 | 3.72 | 1.26 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,4,3,3,3 | lR3HOBut | 175.2 | 46.1 | 66.3 | 23.1 | |
| 3,4,3,3 | aDFucp3N | 96.5 | 67.1 | 52.2 | 71.8 | 68.1 | 16.3 |
| 3,4,3 | aLRhap | 102.5 | 75.8 | 76.1 | 72.1 | 70.6 | 18.0 |
| 3,4 | bLRhap | 101.5 | 71.7 | 81.9 | 72.2 | 73.2 | 18.0 |
| 3 | aLRhap | 103.4 | 71.0 | 70.2 | 83.7 | 68.7 | 18.0 |
| 2 | Ac | 175.4 | 23.6 | |
| | bDFucpN | 103.1 | 52.4 | 81.0 | 71.8 | 71.9 | 16.6 |
|
There is only one chemically distinct structure:
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