Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: pneumonia [ICD11:
CA40 
];
nosocomial infections [ICD11:
XB25 ];
urinary tract infections (UTI) [ICD11:
GC08 ];
septicemia [ICD11:
MA15.Y ];
infection due to Klebsiella pneumoniae [ICD11:
XN741 ]
NCBI PubMed ID: 26493302Publication DOI: 10.1038/srep15573Journal NLM ID: 101563288Publisher: London: Nature Publishing Group
Correspondence: wangjt
ntu.edu.tw
Institutions: Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan
A total of 79 capsular types have been reported in Klebsiella spp., whereas capsular polysaccharide synthesis (cps) regions were available in only 22 types. Due to the limitations of serotyping, complete repertoire of cps will be helpful for capsular genotyping. We therefore resolved the rest 57 cps and conducted comparative analysis. Clustering results of 1,515 predicted proteins from cps loci categorized proteins which share similarity into homology groups (HGs) revealing that 77 Wzy polymerases were classified into 56 HGs, which indicate the high specificity of wzy between different types. Accordingly, wzy-based capsular genotyping could differentiate capsule types except for those lacking wzy (K29 and K50), those sharing identical wzy (K22 vs. K37); and should be carefully applied in those exhibited high similarity (K12 vs. K41, K2 vs. K13, K74 vs. K80, K79 vs. KN1 and K30 vs. K69). Comparison of CPS structures in several capsular types that shared similarity in their gene contents implies possible functions of glycosyltransferases. Therefore, our results provide complete set of cps in various types of Klebsiella spp., which enable the understandings of relationship between genes and CPS structures and are useful for identification of documented or new capsular types.
synthesis, structure, capsular polysaccharide, gene cluster, glycosyltransferases, Klebsiella, capsule, serotyping, Wzy polymerase, genotyping
Structure type: polymer chemical repeating unit
Location inside paper: fig.S1b, K61
Compound class: CPS, K-antigen
Contained glycoepitopes: IEDB_115136,IEDB_130701,IEDB_136906,IEDB_137472,IEDB_140630,IEDB_141794,IEDB_141806,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_423153,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_7,SB_72
Methods: PCR, DNA sequencing, gene annotation
Related record ID(s): 1085, 1090, 1092, 1133, 2221, 2222, 2223, 2224, 2225, 2226, 2227, 2228, 2229, 2230, 2231, 2233, 2234
NCBI Taxonomy refs (TaxIDs): 573Reference(s) to other database(s): GTC:G67194AM, GlycomeDB:
4930
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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