Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 37894721Publication DOI: 10.3390/ijms242015040Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: J. Hu <hujing
jiangnan.edu.cn>; X. Guo <guoxi
nankai.edu.cn>
Institutions: TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin 300457, China, Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Lihu Ave. 1800, Wuxi 214122, China, Wuxi School of Medicine, Jiangnan University, Lihu Ave. 1800, Wuxi 214122, China
The O-antigen is one of the outermost surface components of Gram-negative bacteria. Its large structural variation provides the molecular basis for bacterial serological diversity. Here, we established the structure of the O-antigen from an Escherichia coli strain, SD2019180, which appeared to be completely different from the known E. coli serogroups. The O-antigen tetrasaccharide biological repeating unit was identified as →2)-[β-d-GlcpA-(1→4)]-[α-d-Galp-(1→3)]-α-l-Fucp-(1→3)-α-d-GlcpNAc-(1→. Furthermore, we analyzed the O-antigen gene cluster of SD2019180 and confirmed its role in O-antigen synthesis by using deletion and complementation experiments. Our findings indicate that SD2019180 is a novel serogroup of Escherichia coli.
structure, O-antigen, Escherichia coli, serogroup, O-antigen gene cluster
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 8, table 1
Compound class: O-antigen
Contained glycoepitopes: IEDB_115136,IEDB_136045,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_140630,IEDB_141794,IEDB_141807,IEDB_142489,IEDB_144562,IEDB_145669,IEDB_151528,IEDB_151531,IEDB_152214,IEDB_174333,IEDB_190606,IEDB_423153,SB_7,SB_86
Methods: 13C NMR, 1H NMR, NMR-2D, SDS-PAGE, sugar analysis, HPLC, HPSEC, HPAEC-PAD, genome sequencing, annotation
NCBI Taxonomy refs (TaxIDs): 562
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3 aDGalp 100.0 68.9 69.2 69.6 71.3 61.7
3,4 bDGlcpA 103.7 73.3 75.2 71.3 75.8 169.0
3 aLFucp 99.8 67.9 73.9 80.1 67.5 15.2
2 Ac 174.5 22.2
aDGlcpN 97.3 53.1 78.0 68.3 72.2 60.4
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3 aDGalp 5.23 3.74 3.82 3.69 4.06 3.68-3.70
3,4 bDGlcpA 4.65 3.45 3.57 3.63 3.98 -
3 aLFucp 5.05 3.95 4.02 4.14 4.42 1.30
2 Ac - 2.04
aDGlcpN 4.78 4.10 3.77 3.55 3.67 3.80-3.87
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3 aDGalp 100.0/5.23 68.9/3.74 69.2/3.82 69.6/3.69 71.3/4.06 61.7/3.68-3.70
3,4 bDGlcpA 103.7/4.65 73.3/3.45 75.2/3.57 71.3/3.63 75.8/3.98
3 aLFucp 99.8/5.05 67.9/3.95 73.9/4.02 80.1/4.14 67.5/4.42 15.2/1.30
2 Ac 22.2/2.04
aDGlcpN 97.3/4.78 53.1/4.10 78.0/3.77 68.3/3.55 72.2/3.67 60.4/3.80-3.87
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3 | aDGalp | 5.23 | 3.74 | 3.82 | 3.69 | 4.06 | 3.68
3.70 |
| 3,4 | bDGlcpA | 4.65 | 3.45 | 3.57 | 3.63 | 3.98 |
|
| 3 | aLFucp | 5.05 | 3.95 | 4.02 | 4.14 | 4.42 | 1.30 |
| 2 | Ac |
| 2.04 | |
| | aDGlcpN | 4.78 | 4.10 | 3.77 | 3.55 | 3.67 | 3.80
3.87 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3 | aDGalp | 100.0 | 68.9 | 69.2 | 69.6 | 71.3 | 61.7 |
| 3,4 | bDGlcpA | 103.7 | 73.3 | 75.2 | 71.3 | 75.8 | 169.0 |
| 3 | aLFucp | 99.8 | 67.9 | 73.9 | 80.1 | 67.5 | 15.2 |
| 2 | Ac | 174.5 | 22.2 | |
| | aDGlcpN | 97.3 | 53.1 | 78.0 | 68.3 | 72.2 | 60.4 |
|
There is only one chemically distinct structure:
Found 
report error