Perepelov AV, Li X, Xu C, Filatov AV, Shashkov AS, Senchenkova SN, Liu B Structure elucidation and gene cluster characterization of the O-antigen of Vibrio cholerae O14 Carbohydrate Research474 (2019)
67-71
The structure was elucidated in this paper NCBI PubMed ID:30763794 Publication DOI:10.1016/j.carres.2019.01.007 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: A.V. Perepelov <andreivperepelovgmail.com> Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, TEDA Institure of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China
The O-polysaccharide (O-antigen) of Vibrio cholerae O14 was studied using chemical analyses and 1D and 2D NMR spectroscopy. The following structure of the repeating unit of the O-antigen was established: where GlcpN(SHb) indicates 2-deoxy-2-[(S)-3-hydroxybutanoylamino]-d-glucose. We found that Vibrio cholerae O14 is similar to that of O-polysaccharide of Azospirillum brasilense S17, which has been reported earlier. Moreover, we predicted functions of all the genes in the O-antigen gene cluster according to the structure established. Our study enriches the existing O-antigen database of Vibrio cholerae, and further facilitates the bacterial serotype identification.
Methods: 13C NMR, 1H NMR, NMR-2D, DNA sequencing, sugar analysis, acid hydrolysis, GLC, Smith degradation, GPC, delipidation, function analysis of gene clusters, function analysis of gene cluster, 13C NMR analysis by GRASS Comments, role: V. cholerae O14 type strain (laboratory stock number G5476). Smith degraded OPS.
Related record ID(s): 1089, 2256, 2258 NCBI Taxonomy refs (TaxIDs):666 Reference(s) to other database(s): GlycomeDB:3416 Show glycosyltransferases
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