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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Campylobacter jejuni [ICD11: XN4Q5

]

NCBI PubMed ID: 18156268
Journal NLM ID: 2985120R
Publisher: American Society for Microbiology
Correspondence: patricia.guerry

med.navy.mil
Institutions: Department of Chemistry, North Carolina Agricultural and Technical State University, Greensboro, North Carolina 27411, USA

We report isolation and characterization of Campylobacter jejuni 81-176 lgtF and galT lipooligosaccharide (LOS) core mutants. It has been suggested that the lgtF gene of C. jejuni encodes a two-domain glucosyltransferase that is responsible for the transfer of a β-1,4-glucose residue on heptosyltransferase I (Hep I) and for the transfer of a β-1,2-glucose residue on Hep II. A site-specific mutation in the lgtF gene of C. jejuni 81-176 resulted in expression of a truncated LOS, and complementation of the mutant in trans restored the core mobility to that of the wild type. Mass spectrometry and nuclear magnetic resonance of the truncated LOS confirmed the loss of two glucose residues, a β-1,4-glucose on Hep I and a β-1,2-glucose on Hep II. Mutation of another gene, galT, encoding a glycosyltransferase, which maps outside the region defined as the LOS biosynthetic locus in C. jejuni 81-176, resulted in loss of the β-(1,4)-galactose residue and all distal residues in the core. Both mutants invaded intestinal epithelial cells in vitro at levels comparable to the wild-type levels, in marked contrast to a deeper inner core waaC mutant. These studies have important implications for the role of LOS in the pathogenesis of Campylobacter-mediated infection

biosynthesis, core, Lipooligosaccharide, Campylobacter jejuni, mutants, glycosyltransferase

Structure type: oligomer
Location inside paper: p. 1569, fig. 1A
Compound class: LOS, LPS
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130648,IEDB_130650,IEDB_130679,IEDB_130683,IEDB_136044,IEDB_136794,IEDB_137472,IEDB_137473,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_153778,IEDB_1625329,IEDB_190606,IEDB_2189047,IEDB_983931,SB_116,SB_144,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_25,SB_37,SB_39,SB_6,SB_68,SB_7,SB_76,SB_84,SB_88

Methods: 13C NMR, 1H NMR, GLC-MS, GC-MS, sugar analysis, 31P NMR, ESI-MS, acid hydrolysis, genetic methods
Biosynthesis and genetic data: genetic data

Related record ID(s): 22996, 22997
NCBI Taxonomy refs (TaxIDs): 354242
Reference(s) to other database(s): GTC:G59882DP
Show glycosyltransferases

There are 2 chemically distinct structures. Please, select:

CC(=O)N[C@H]1[C@H](O[C@H]2[C@@H](CO)O[C@@H](O[C@@H]3[C@@H](CO)O[C@@H](O[C@H]4[C@@H](O)[C@H](O)[C@@H]([C@@H](O)CO)O[C@@H]4O[C@@H]4[C@H](O)[C@@H](O[C@@H]5[C@H](O)CC(O)(C(=O)O)O[C@@H]5[C@H](O)CO)O[C@H]([C@H](CO)OP(=O)(O)OCCN)[C@H]4O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O[C@]2(C(=O)O)C[C@H](O)[C@@H](NC(C)=O)[C@H]([C@H](O)[C@H](O)CO)O2)O[C@H](CO)[C@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize