Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria) Host organism: Homo sapiens
Organ / tissue: middle ear Associated disease: acute otitis media [ICD11:
AB00 , Life stage: adult];
infection due to Haemophilus influenzae [ICD11:
XN1P6 ]
NCBI PubMed ID: 18458064 Journal NLM ID: 0246127 Publisher: American Society for Microbiology
Correspondence: elke.schweda
crc.ki.se
Institutions: Karolinska Institutet and University College of South Stockholm, Clinical Research Center, Huddinge, Sweden
Otitis media caused by nontypeable Haemophilus influenzae (NTHi) is a common and recurrent bacterial infection of childhood. The structural variability and diversity of H. influenzae lipopolysaccharide (LPS) glycoforms are known to play a significant role in the commensal and disease-causing behavior of this pathogen. In this study, we determined LPS glycoform populations from NTHi strain 1003 during the course of experimental otitis media in the chinchilla model of infection by mass spectrometric techniques. Building on an established structural model of the major LPS glycoforms expressed by this NTHi strain in vitro (M. Mansson, W. Hood, J. Li, J. C. Richards, E. R. Moxon, and E. K. Schweda, Eur. J. Biochem. 269:808-818, 2002), minor isomeric glycoform populations were determined by liquid chromatography multiple-step tandem electrospray mass spectrometry (LC-ESI-MS(n)). Using capillary electrophoresis ESI-MS (CE-ESI-MS), we determined glycoform profiles for bacteria from direct middle ear fluid (MEF) samples. The LPS glycan profiles were essentially the same when the MEF samples of 7 of 10 animals were passaged on solid medium (chocolate agar). LC-ESI-MS(n) provided a sensitive method for determining the isomeric distribution of LPS glycoforms in MEF and passaged specimens. To investigate changes in LPS glycoform distribution during the course of infection, MEF samples were analyzed at 2, 5, and 9 days postinfection by CE-ESI-MS following minimal passage on chocolate agar. As previously observed, sialic acid-containing glycoforms were detected during the early stages of infection, but a trend toward more-truncated and less-complex LPS glycoforms that lacked sialic acid was found as disease progressed
Haemophilus influenzae, lipopolysaccharides, LPS, ESI-MS, Disease Models, otitis media, capillary electrophoresis, liquid chromatography, electrospray mass spectrometry
Structure type: oligomer
Location inside paper: p.3256, fig.1
Aglycon: lipid A
Compound class: LPS
Contained glycoepitopes: IEDB_115009,IEDB_116046,IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130679,IEDB_136044,IEDB_136794,IEDB_137472,IEDB_137777,IEDB_137779,IEDB_138949,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_140624,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_190606,IEDB_2189047,IEDB_241118,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_37,SB_39,SB_6,SB_61,SB_68,SB_7,SB_76,SB_84,SB_88
Methods: methylation, HF solvolysis, ESI-MS, mild acid hydrolysis, alkaline hydrolysis, CE-ESI-MS, LC-ESI-MS
Biological activity: Experimental otitis media model
NCBI Taxonomy refs (TaxIDs): 727
Show glycosyltransferases
There are 36 chemically distinct structures. Please, select:
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-2)]bDGlcp(1-2)[xXGly?(1-4),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-1),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-3)]bDGlcp(1-2)[xXGly?(1-4),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-1),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-6)]bDGlcp(1-2)[xXGly?(1-4),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-1),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-2)]bDGlcp(1-2)[xXGly?(1-6),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-1),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-3)]bDGlcp(1-2)[xXGly?(1-6),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-1),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-6)]bDGlcp(1-2)[xXGly?(1-6),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-1),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-2)]bDGlcp(1-2)[xXGly?(1-7),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-1),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-3)]bDGlcp(1-2)[xXGly?(1-7),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-1),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-6)]bDGlcp(1-2)[xXGly?(1-7),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-1),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-2)]bDGlcp(1-2)[xXGly?(1-4),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-2),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-3)]bDGlcp(1-2)[xXGly?(1-4),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-2),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-6)]bDGlcp(1-2)[xXGly?(1-4),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-2),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-2)]bDGlcp(1-2)[xXGly?(1-6),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-2),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-3)]bDGlcp(1-2)[xXGly?(1-6),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-2),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-6)]bDGlcp(1-2)[xXGly?(1-6),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-2),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-2)]bDGlcp(1-2)[xXGly?(1-7),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-2),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-3)]bDGlcp(1-2)[xXGly?(1-7),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-2),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-6)]bDGlcp(1-2)[xXGly?(1-7),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-2),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-2)]bDGlcp(1-2)[xXGly?(1-4),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-7),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-3)]bDGlcp(1-2)[xXGly?(1-4),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-7),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-6)]bDGlcp(1-2)[xXGly?(1-4),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-7),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-2)]bDGlcp(1-2)[xXGly?(1-6),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-7),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-3)]bDGlcp(1-2)[xXGly?(1-6),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-7),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-6)]bDGlcp(1-2)[xXGly?(1-6),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-7),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-2)]bDGlcp(1-2)[xXGly?(1-7),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-7),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-3)]bDGlcp(1-2)[xXGly?(1-7),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-7),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-6)]bDGlcp(1-2)[xXGly?(1-7),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-7),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-2)]bDGlcp(1-2)[xXGly?(1-4),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-8),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-3)]bDGlcp(1-2)[xXGly?(1-4),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-8),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-6)]bDGlcp(1-2)[xXGly?(1-4),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-8),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-2)]bDGlcp(1-2)[xXGly?(1-6),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-8),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-3)]bDGlcp(1-2)[xXGly?(1-6),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-8),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-6)]bDGlcp(1-2)[xXGly?(1-6),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-8),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-2)]bDGlcp(1-2)[xXGly?(1-7),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-8),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-3)]bDGlcp(1-2)[xXGly?(1-7),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-8),xXEt?N(1-P-P-4)]aXKdop
Ac(1-5)aXNeup(2-3)bDGalp(1-4)[Ac(1-6)]bDGlcp(1-2)[xXGly?(1-7),Ac(1-3)]aXLDmanHepp(1-2)[xXEt?N(1-P-6)]aXLDmanHepp(1-3)[Ac(1-4)[xXCho?(1-P-6)]bDGlcp(1-4)]aXLDmanHepp(1-5)[xXGly?(1-8),xXEt?N(1-P-P-4)]aXKdop
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