Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 18062946Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: perepel
ioc.ac.ru
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, RussiaTEDA School of Biological Sciences and Biotechnology, Nankai University, 23 HongDa Street, TEDA, Tianjin, ChinaTianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, 23 HongDa Street, TEDA, Tianjin, China
An acidic O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Escherichia coli O112ab and studied by sugar analysis along with 1H and 13C NMR spectroscopy. The O-polysaccharide was found to contain a rarely occurring sugar component, L-iduronic acid (L-IdoA), and the following structure of the branched pentasaccharide repeating unit was established
O-antigen, Escherichia coli, NMR spectroscopy, O-polysaccharide, bacterial polysaccharide structure, L-iduronic acid
Structure type: suggested polymer biological repeating unit
Location inside paper: p.571, abstract
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_135813,IEDB_136021,IEDB_137340,IEDB_137473,IEDB_1391961,IEDB_1391965,IEDB_141584,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_423113,IEDB_885822,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, GLC-MS, NMR-2D, sugar analysis, GLC, mild acid hydrolysis, alkaline degradation, NMR-1D
Related record ID(s): 23057
NCBI Taxonomy refs (TaxIDs): 2184071Reference(s) to other database(s): GTC:G34491JZ, GlycomeDB:
36883
Show glycosyltransferases
NMR conditions: in D2O at 308 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,4,4,2 Ac 175.0 23.8
3,4,4,3,2 Ac 175.0 23.8
3,4,4,3 bDGlcpN 104.1 57.2 75.2 71.8 77.4 62.5
3,4,4 aDGalpN 99.9 50.2 77.7 75.8 72.3 61.8
3,4 aDGlcp 97.6 73.1 75.2 76.7 72.3 62.2
3 aLIdopA 104.9 70.9 69.7 76.0 69.7 175.0
2 Ac 175.0 23.8
bDGalpN 102.4 52.7 81.0 69.7 76.7 62.6
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,4,2 Ac - 2.07
3,4,4,3,2 Ac - 2.07
3,4,4,3 bDGlcpN 4.55 3.82 3.55 3.44 3.45 3.79-3.94
3,4,4 aDGalpN 5.30 4.20 4.00 4.45 4.06 3.78-3.81
3,4 aDGlcp 5.20 3.50 3.83 3.62 3.75 3.70-3.81
3 aLIdopA 5.04 3.63 3.99 4.15 4.75 -
2 Ac - 2.03
bDGalpN 4.97 4.14 3.86 4.00 3.67 3.75-3.77
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,4,4,2 Ac 23.8/2.07
3,4,4,3,2 Ac 23.8/2.07
3,4,4,3 bDGlcpN 104.1/4.55 57.2/3.82 75.2/3.55 71.8/3.44 77.4/3.45 62.5/3.79-3.94
3,4,4 aDGalpN 99.9/5.30 50.2/4.20 77.7/4.00 75.8/4.45 72.3/4.06 61.8/3.78-3.81
3,4 aDGlcp 97.6/5.20 73.1/3.50 75.2/3.83 76.7/3.62 72.3/3.75 62.2/3.70-3.81
3 aLIdopA 104.9/5.04 70.9/3.63 69.7/3.99 76.0/4.15 69.7/4.75
2 Ac 23.8/2.03
bDGalpN 102.4/4.97 52.7/4.14 81.0/3.86 69.7/4.00 76.7/3.67 62.6/3.75-3.77
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,4,2 | Ac |
| 2.07 | |
| 3,4,4,3,2 | Ac |
| 2.07 | |
| 3,4,4,3 | bDGlcpN | 4.55 | 3.82 | 3.55 | 3.44 | 3.45 | 3.79
3.94 |
| 3,4,4 | aDGalpN | 5.30 | 4.20 | 4.00 | 4.45 | 4.06 | 3.78
3.81 |
| 3,4 | aDGlcp | 5.20 | 3.50 | 3.83 | 3.62 | 3.75 | 3.70
3.81 |
| 3 | aLIdopA | 5.04 | 3.63 | 3.99 | 4.15 | 4.75 |
|
| 2 | Ac |
| 2.03 | |
| | bDGalpN | 4.97 | 4.14 | 3.86 | 4.00 | 3.67 | 3.75
3.77 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,4,4,2 | Ac | 175.0 | 23.8 | |
| 3,4,4,3,2 | Ac | 175.0 | 23.8 | |
| 3,4,4,3 | bDGlcpN | 104.1 | 57.2 | 75.2 | 71.8 | 77.4 | 62.5 |
| 3,4,4 | aDGalpN | 99.9 | 50.2 | 77.7 | 75.8 | 72.3 | 61.8 |
| 3,4 | aDGlcp | 97.6 | 73.1 | 75.2 | 76.7 | 72.3 | 62.2 |
| 3 | aLIdopA | 104.9 | 70.9 | 69.7 | 76.0 | 69.7 | 175.0 |
| 2 | Ac | 175.0 | 23.8 | |
| | bDGalpN | 102.4 | 52.7 | 81.0 | 69.7 | 76.7 | 62.6 |
|
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
