Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 18700154Journal NLM ID: 8306883Correspondence: wanglei
nankai.edu.cn (L. Wang)
Institutions: TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 HongDa Street, TEDA, Tianjin, China, Tianjin Key Laboratory of Microbial Functional Genomics, 23 Hongda Street, TEDA, Tianjin, China
Escherichia coli O3 and O21 are associated with enteroaggregative E. coli (EAEC). EAEC strains are often non-typable using the routine agglutination method due to their aggregative phenotype. Typing of E. coli O3 and O21 may also be impeded by cross-reactions with O152 or O83. In this study, the O antigen gene clusters of E. coli O3 and O21 were characterized, and PCR assays based on O antigen specific genes wzx (encoding O unit flippase) and wzy (encoding O unit polymerase) from each strain were developed. By screening against all 186 known E. coli O serotypes, the PCR assays were shown to be highly specific to O3 and O21 respectively. The sensitivity of the assays was determined to be 1 pg per mul of chromosomal DNA and 2 CFU per 10 g of water samples. The PCR assays were also applied to 658 clinical E. coli isolates, and 100% of detection accuracy was obtained. The PCR assays developed here are suitable for the detection and identification of E. coli O3 and O21 strains in environmental and clinical samples
PCR, O antigen, O-serotype, wzx, wzy, E.coli 03, E.coli 021
Structure type: suggested polymer biological repeating unit
Location inside paper: p. 332, fig. 2
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_983931,SB_173,SB_192,SB_7
Methods: PCR, genetic methods
Biosynthesis and genetic data: genetic data
Comments, role: Corrected structure, the structure in the paper was erroneous. Biological repeat frame was based on [PMID:27196310]
Related record ID(s): 10304, 11291, 20634, 23242
NCBI Taxonomy refs (TaxIDs): 2234097Reference(s) to other database(s): GTC:G44739ZN, GlycomeDB:
28095
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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