Taxonomic group: bacteria / Actinobacteria
(Phylum: Actinobacteria)
Associated disease: infection due to Mycobacterium tuberculosis [ICD11:
XN1N2 
]
The structure was elucidated in this paperNCBI PubMed ID: 18808383Journal NLM ID: 8712028Publisher: Blackwell Publishing
Correspondence: mamadou.daffe
ipbs.fr; Mary.Jackson
ColoState.edu
Institutions: Universite Paul Sabatier (Toulouse III) 118, route de Narbonne, 31062-Toulouse cedex 04, France
Mycobacterium tuberculosis and other pathogenic mycobacterial species produce large amounts of a glycogen-like α-glucan that represents the major polysaccharide of their outermost capsular layer. To determine the role of the surface-exposed glucan in the physiology and virulence of these bacteria, orthologues of the glg genes involved in the biosynthesis of glycogen in Escherichia coli were identified in M. tuberculosis H37Rv and inactivated by allelic replacement. Biochemical analyses of the mutants and complemented strains indicated that the synthesis of glucan and glycogen involves the α-1,4-glucosyltransferases Rv3032 and GlgA (Rv1212c), the ADP-glucose pyrophosphorylase GlgC (Rv1213) and the branching enzyme GlgB (Rv1326c). Disruption of glgC reduced by half the glucan and glycogen contents of M. tuberculosis, whereas the inactivation of glgA and Rv3032 affected the production of capsular glucan and glycogen, respectively. Attempts to disrupt Rv3032 in the glgA mutant were unsuccessful, suggesting that a functional copy of at least one of the two α-1,4-glucosyltransferases is required for growth. Importantly, the glgA mutant was impaired in its ability to persist in mice, suggesting a role for the capsular glucan in the persistence phase of infection. Unexpectedly, GlgB was found to be an essential enzyme
biosynthesis, gene, Mycobacterium tuberculosis, tuberculosis, a-glucan, glgA mutant
Structure type: homopolymer
Location inside paper: p. 763
Trivial name: methyl glucose lipopolysaccharide, glucan, maltosaccharide, α-1,4-D-glucan, amylose, α-glucan, glycogen backbone, α-(1,4)-glucan, starch, α-(1-4)-glucan, starch, glycogen
Compound class: CPS, EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, methyl glucose lipopolysaccharide
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, GC-MS, GC, MALDI-TOF MS, genetic methods
Biosynthesis and genetic data: genetic data
3D data: conformation data
Related record ID(s): 913, 8387, 10122, 23997, 102751, 106369
NCBI Taxonomy refs (TaxIDs): 1773Reference(s) to other database(s): GTC:G05740LL, GlycomeDB:
12100, CCSD:
4943, CBank-STR:819
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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