Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 18047581Journal NLM ID: 8712028Publisher: Blackwell Publishing
Correspondence: dominique.mengin-lecreulx
u-psud.fr
Institutions: Laboratoire des Enveloppes Bactériennes et Antibiotiques, Unité Mixte de Recherche 8619 CNRS, Université Paris-Sud, Orsay, France, Department of Microbiology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN, USA, Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, GA, USA
One-third of the lipid A found in the Escherichia coli outer membrane contains an unsubstituted diphosphate unit at position 1 (lipid A 1-diphosphate). We now report an inner membrane enzyme, LpxT (YeiU), which specifically transfers a phosphate group to lipid A, forming the 1-diphosphate species. (32)P-labelled lipid A obtained from lpxT mutants do not produce lipid A 1-diphosphate. In vitro assays with Kdo(2)-[4'-(32)P]lipid A as the acceptor shows that LpxT uses undecaprenyl pyrophosphate as the substrate donor. Inhibition of lipid A 1-diphosphate formation in wild-type bacteria was demonstrated by sequestering undecaprenyl pyrophosphate with the cyclic polypeptide antibiotic bacitracin, providing evidence that undecaprenyl pyrophosphate serves as the donor substrate within whole bacteria. LpxT-catalysed phosphorylation is dependent upon transport of lipid A across the inner membrane by MsbA, a lipid A flippase, indicating a periplasmic active site. In conclusion, we demonstrate a novel pathway in the periplasmic modification of lipid A that is directly linked to the synthesis of undecaprenyl phosphate, an essential carrier lipid required for the synthesis of various bacterial polymers, such as peptidoglycan.
Escherichia coli, lipid A, Undecaprenyl phosphate, LpxT
Structure type: oligomer
Location inside paper: p.25806, fig.1, Kdo2-lipid A 1,4'-bis-phosphorylated
Trivial name: inner core, Kdo2-lipid A
Compound class: core oligosaccharide, LOS, lipid A
Contained glycoepitopes: IEDB_130650,IEDB_130659,IEDB_135394,IEDB_135515,IEDB_141807,IEDB_150908,IEDB_151531,IEDB_176772,IEDB_534864
Methods: SDS-PAGE, DNA techniques, TLC, genetic methods, biochemical methods, enzyme assay
Biosynthesis and genetic data: genetic data
Related record ID(s): 31252
NCBI Taxonomy refs (TaxIDs): 83333
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There is only one chemically distinct structure:
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