Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: melioidosis [ICD11:
1C42 
, ICD11:
XN3LD ];
infection due to Burkholderia pseudomallei [ICD11:
XN3LD ];
cystic fibrosis (CF) [ICD11:
CA25 ]
The structure was elucidated in this paperNCBI PubMed ID: 31548320Publication DOI: 10.1128/IAI.00692-19Journal NLM ID: 0246127Publisher: American Society for Microbiology
Correspondence: narisara
tropmedres.ac
Institutions: Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, Department of Microbial Pathogenesis, University of Maryland, Baltimore, Maryland, USA, Division of Pulmonary and Critical Care Medicine, Harborview Medical Center, University of Washington, Seattle, Washington, USA, International Respiratory and Severe Illness Center, University of Washington, Seattle, Washington, USA
Lipopolysaccharides (LPSs) of Gram-negative bacteria comprise lipid A, core, and O-polysaccharide (OPS) components. Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators. The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities. Here, we describe the structural and immunological characteristics of the LPSs isolated from eight Burkholderia species and compare those for B. pseudomallei to those for the other seven species. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species. MALDI-TOF profiles demonstrated that Burkholderia lipid A contains predominantly penta-acylated species modified with 4-amino-4-deoxy-arabinose residues at both terminal phosphate groups. The lipid A could be differentiated based on mass differences at m/z 1,511, 1,642, 1,773, and 1,926 and on fatty acid composition. LPSs of all species induced TLR4-dependent NF-?B responses; however, while SDS-PAGE analysis showed similar LPS ladder patterns for B. pseudomallei, B. thailandensis, and B. mallei, these patterns differed from those of other Burkholderia species. Interestingly, immunoblot analysis demonstrated that melioidosis patient sera cross-reacted with OPSs of other Burkholderia species. These findings can be used to better understand the characteristics of LPS in Burkholderia species, and they have implications for serological diagnostics based on the detection of antibodies to OPS.
Lipopolysaccharide, Burkholderia, antibody, O-polysaccharide, serology, melioidosis, innate immunity, LPS structure, TLR4, Diagnostics, bacterial infection
Structure type: oligomer ; 1686
Location inside paper: fig.3, pentaacylated lipid A
Compound class: lipid A
Contained glycoepitopes: IEDB_135394,IEDB_135515,IEDB_141807,IEDB_151531
Methods: SDS-PAGE, GC, Western blotting, chemical methods, MALDI-TOF MS, serological methods, statistical analysis, TLR4 activation assay
Related record ID(s): 1103, 2276, 2277, 2278, 2279, 2280
NCBI Taxonomy refs (TaxIDs): 272560,
87883,
60552
Show glycosyltransferases
There is only one chemically distinct structure:
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