Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: plague [ICD11:
1B93 
, ICD11:
XN6QS ];
infection due to Yersinia pestis [ICD11:
XN6QS ]
The structure was elucidated in this paperNCBI PubMed ID: 31228931Publication DOI: 10.1134/S0006297919040072Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Correspondence: S. V. Dentovskaya <dentovskaya
obolensk.org>
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russia
To identify Yersinia pestis genes involved in the microbe's resistance to cationic antimicrobial peptides, the strategy of random transposon mutagenesis with a Tn5 minitransposon was used, and the library was screened for detecting polymyxin B (PMB) susceptible mutants. The mutation responsible for PMB-sensitive phenotype and the lipopolysaccharide (LPS) structure were characterized for the Y. pestis strain KM218-A3. In this strain the mini-Tn5 was located in an open reading frame with the product homologous to the E. coli protein GmhB (82% identity) functioning as d-glycero-d-manno-heptose-1,7-diphosphate phosphatase. ESI FT ICR mass spectrometry of anions was used to study the structure of the unmodified LPS of Y. pestis KM218-A3, and molecules were revealed with the full-size LPS core or with two types of an incomplete core: consisting of Kdo-Kdo or Ko-Kdo disaccharides and Hep-(Kdo)-Kdo or Hep-(Ko)-Kdo trisaccharides. The performed complementation confirmed that the defect in the biological properties of the mutant strain was caused by inactivation of the gmhB gene. These findings indicated that the gmhB gene product of Y. pestis is essential for production of wild-type LPS resistant to antimicrobial peptides and serum.
Lipopolysaccharide, serum resistance, heptose biosynthesis, Yersinia pestis, antibiotic resistance
Structure type: oligomer
Location inside paper: p.401, table 3, core type 3c
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_135607,IEDB_135609,IEDB_2189047
Methods: DNA sequencing, SDS-PAGE, DNA techniques, ESI-ICR-MS, serological methods, insertion mutagenesis, complementation
Comments, role: Yersinia pestis mutant strain KM218-A3; major glycoform
Related record ID(s): 1105, 2281, 2282, 2283, 2284, 2285, 2286, 2288, 2289, 2290
NCBI Taxonomy refs (TaxIDs): 632Reference(s) to other database(s): GTC:G23389YG
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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