Kanipes MI, Tan X, Akelaitis A, Li J, Rockabrand D, Guerry P, Monteiro MA Genetic analysis of lipooligosaccharide core biosynthesis in Campylobacter jejuni 81-176 Journal of Bacteriology190(5) (2008)
1568-1574
The structure was elucidated in this paper NCBI PubMed ID:18156268 Journal NLM ID:2985120R Publisher: American Society for Microbiology Correspondence: patricia.guerrymed.navy.mil Institutions: Department of Chemistry, North Carolina Agricultural and Technical State University, Greensboro, North Carolina 27411, USA
We report isolation and characterization of Campylobacter jejuni 81-176 lgtF and galT lipooligosaccharide (LOS) core mutants. It has been suggested that the lgtF gene of C. jejuni encodes a two-domain glucosyltransferase that is responsible for the transfer of a β-1,4-glucose residue on heptosyltransferase I (Hep I) and for the transfer of a β-1,2-glucose residue on Hep II. A site-specific mutation in the lgtF gene of C. jejuni 81-176 resulted in expression of a truncated LOS, and complementation of the mutant in trans restored the core mobility to that of the wild type. Mass spectrometry and nuclear magnetic resonance of the truncated LOS confirmed the loss of two glucose residues, a β-1,4-glucose on Hep I and a β-1,2-glucose on Hep II. Mutation of another gene, galT, encoding a glycosyltransferase, which maps outside the region defined as the LOS biosynthetic locus in C. jejuni 81-176, resulted in loss of the β-(1,4)-galactose residue and all distal residues in the core. Both mutants invaded intestinal epithelial cells in vitro at levels comparable to the wild-type levels, in marked contrast to a deeper inner core waaC mutant. These studies have important implications for the role of LOS in the pathogenesis of Campylobacter-mediated infection
Methods: 13C NMR, 1H NMR, GLC-MS, GC-MS, sugar analysis, 31P NMR, ESI-MS, acid hydrolysis, genetic methods Enzymes that release or process the structure: lgtF Biosynthesis and genetic data: genetic data
Related record ID(s): 22662, 22996 NCBI Taxonomy refs (TaxIDs):354242 Reference(s) to other database(s): GTC:G75856GL Show glycosyltransferases
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(later renamed to: Campylobacter jejuni subsp. jejuni 81-176 lgtF)
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