CSDB: Search results

Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: respiratory infections [ICD11: CA45

]; infection due to Bordetella bronchiseptica [ICD11: XN173

]

NCBI PubMed ID: 18539367
Publication DOI: 10.1016/j.vaccine.2008.04.079
Journal NLM ID: 8406899
Publisher: Elsevier
Correspondence: J. Kubler-Kielb <kielbj

mail.nih.gov>
Institutions: National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD, USA

Bordetellae are Gram-negative bacilli causing respiratory tract infections of mammals and birds. Clinically important are B. pertussis, B. parapertussis and B. bronchiseptica. B. pertussis vaccines have been successful in preventing pertussis in infants and children. Veterinary vaccines against B. bronchiseptica are available, but their efficacy and mode of action are not established. There is no vaccine against B. parapertussis. Based on the concept that immunity to non-capsulated Gram-negative bacteria may be conferred by serum IgG anti-LPS we studied chemical, serological and immunological properties of the O-specific polysaccharides (O-SP) of B. bronchiseptica and B. parapertussis obtained by different degradation procedures. One type of the B. parapertussis and two types of B. bronchiseptica O-SP were recognized based on the structure of their non-reducing end saccharide; no cross-reaction between the two B. bronchiseptica types was observed. Competitive inhibition assays showed the immunodominance of the non-reducing end of these O-SP. Conjugates of B. bronchiseptica and B. parapertussis O-SP were prepared by two methods: using the anhydro-Kdo residue exposed by mild acid hydrolysis of the LPS or the 2,5-anhydromannose residue exposed by deamination of the core glucosamine of the LPS, for binding to an aminooxylated protein. Both coupling methods were carried out at a neutral pH, room temperature, and in a short time. All conjugates, injected as saline solutions at a fraction of an estimated human dose, induced antibodies in mice to the homologous O-SP. These methodologies can be applied to prepare O-SP-based vaccines against other Gram-negative bacteria

LPS, Bordetella, O-specific polysaccharides, vaccine, conjugate

Structure type: oligomer
Location inside paper: p.3590, fig. 3
Aglycon: (2->6)lipid A
Compound class: LPS
Contained glycoepitopes: IEDB_115136,IEDB_130650,IEDB_137340,IEDB_137777,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_140630,IEDB_141807,IEDB_142345,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_2189047,IEDB_2275071,IEDB_983931,SB_192

Methods: HF solvolysis, SDS-PAGE, acid hydrolysis, MALDI-TOF MS, serological methods
Biological activity: serological data, biological activity data

Related record ID(s): 22679, 23030, 23031
NCBI Taxonomy refs (TaxIDs): 518
Reference(s) to other database(s): GTC:G96646TG
Show glycosyltransferases

There is only one chemically distinct structure:

CN[C@@H]1[C@H](O[C@@H]2O[C@H](C(=O)O)[C@@H](O[C@H]3O[C@H](CO[C@@H]4O[C@@H](C(=O)O)[C@@H](O[C@@H]5O[C@@H](C(=O)O)[C@@H](O[C@@H]6O[C@@H](C(=O)O)[C@@H](O[C@@H]7O[C@@H](C(=O)O)[C@@H](O)[C@@H](NC(C)=O)[C@@H]7NC(C)=O)[C@@H](NC(C)=O)[C@@H]6NC(C)=O)[C@@H](NC(C)=O)[C@@H]5NC(C)=O)[C@@H](NC(C)=O)[C@@H]4NC(C)=O)[C@@H](O)[C@H](O)[C@H]3NC(C)=O)[C@H](NC(C)=O)[C@@H]2NC(C)=O)[C@@H](NC(C)=O)[C@H](OC[C@H]2O[C@H](O[C@@H]3[C@@H](CO[C@H]4O[C@H](C(=O)O)[C@H](O)[C@H](O)[C@H]4N)O[C@@H](O[C@@H]4[C@@H]([C@@H](O)CO)O[C@H](O[C@@H]5[C@H](OP(=O)(O)O)CC(O)(C(=O)O)O[C@@H]5[C@H](O)CO)[C@@H](O)[C@H]4O[C@H]4O[C@H]([C@@H](O)CO[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5N)[C@@H](OP(=O)(O)O)[C@H](O)[C@@H]4O[C@H]4O[C@H](C(=O)O)[C@@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@H](N)[C@@H](O)[C@@H]2O[C@H]2O[C@H]([C@@H](O)CO)[C@@H](O)[C@H](O)[C@@H]2O)O[C@@H]1C

SVG MW SMILES 3D mol Ionize

Warning = sub-repeat ending at aLGalpN3NA: unknown unit count was assumed to be 3