Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Organ / tissue: cell wallAssociated disease: infection due to Bacillus anthracis [ICD11:
XN94F 
]
NCBI PubMed ID: 18757856Publication DOI: 10.1074/jbc.M803234200Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: rcarlson
ccrc.uga.edu
Institutions: Complex Carbohydrate Research Center, The University of Georgia, Athens, GA 30605
Non-classical secondary cell wall polysaccharides constitute a major cell wall structure in the Bacillus cereus group of bacteria. The structure of the secondary cell wall polysaccharide from Bacillus cereus ATCC 10987, a strain that is closely related to B. anthracis, was determined. This polysaccharide was released from the cell wall with aqueous hydrogen fluoride (HF) and purified by gel filtration chromatography. The purified polysaccharide, HF-PS, was characterized by glycosyl composition and linkage analyses, mass spectrometry, and 1- and 2-D NMR analysis. The results showed that the B. cereus ATCC 10987 HF-PS has a repeating oligosaccharide consisting of a 6)--GalNAc-(14)--ManNAc-(14)--GlcNAc-(1 trisaccharide that is substituted with -Gal at O3 of the -GalNAc residue, and non-stoichiometrically acetylated at O3 of the ManNAc residue. Comparison of this structure with that of the B. anthracis HF-PS and with structural data obtained for the HF-PS from B. cereus type strain ATCC 14579 revealed that each HF-PS had the same general structural theme consisting of three HexNAc and one Hex residues. A common structural feature in the HF-PSs from B. cereus ATCC 10987 and B. anthracis was the presence of a repeating unit consisting of a HexNAc3 trisaccharide backbone in which two of the three HexNAc residues are GlcNAc and ManNAc, and the third can be either GlcNAc or GalNAc. The implications of these results with regard to the possible functions of the HF-PSs are discussed
structure, repeating unit, Bacillus, cell wall polysaccharide, Bacillus cereus
Structure type: polymer chemical repeating unit
Location inside paper: p.29819, fig.7
Trivial name: HF-PS
Compound class: cell wall polysaccharide
Contained glycoepitopes: IEDB_135813,IEDB_136044,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_140108,IEDB_141794,IEDB_141807,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_885813,SB_165,SB_166,SB_187,SB_195,SB_30,SB_7,SB_88
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, HF solvolysis, 31P NMR, MALDI-TOF MS, composition analysis, NMR-1D, mild hydrazinolysis
Comments, role: HF-PS
Related record ID(s): 22688, 23117
NCBI Taxonomy refs (TaxIDs): 198094,
260799,
1392Reference(s) to other database(s): GTC:G17290CI, GlycomeDB:
37286
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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