Loeff C, Choudhury B, Saile E, Quinn CP, Carlson RW, Kannenberg EL Structural elucidation of the non-classical secondary cell wall polysaccharide from Bacillus cereus ATCC 10987 comparison with the polysaccharides from B. anthracis and B. cereus type strain ATCC 14579 reveals both unique and common structural features Journal of Biological Chemistry283(44) (2008)
2981-2921
The structure was elucidated in this paper NCBI PubMed ID:18757856 Publication DOI:10.1074/jbc.M803234200 Journal NLM ID:2985121R Publisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology Correspondence: rcarlsonccrc.uga.edu Institutions: Complex Carbohydrate Research Center, The University of Georgia, Athens, GA 30605
Non-classical secondary cell wall polysaccharides constitute a major cell wall structure in the Bacillus cereus group of bacteria. The structure of the secondary cell wall polysaccharide from Bacillus cereus ATCC 10987, a strain that is closely related to B. anthracis, was determined. This polysaccharide was released from the cell wall with aqueous hydrogen fluoride (HF) and purified by gel filtration chromatography. The purified polysaccharide, HF-PS, was characterized by glycosyl composition and linkage analyses, mass spectrometry, and 1- and 2-D NMR analysis. The results showed that the B. cereus ATCC 10987 HF-PS has a repeating oligosaccharide consisting of a 6)--GalNAc-(14)--ManNAc-(14)--GlcNAc-(1 trisaccharide that is substituted with -Gal at O3 of the -GalNAc residue, and non-stoichiometrically acetylated at O3 of the ManNAc residue. Comparison of this structure with that of the B. anthracis HF-PS and with structural data obtained for the HF-PS from B. cereus type strain ATCC 14579 revealed that each HF-PS had the same general structural theme consisting of three HexNAc and one Hex residues. A common structural feature in the HF-PSs from B. cereus ATCC 10987 and B. anthracis was the presence of a repeating unit consisting of a HexNAc3 trisaccharide backbone in which two of the three HexNAc residues are GlcNAc and ManNAc, and the third can be either GlcNAc or GalNAc. The implications of these results with regard to the possible functions of the HF-PSs are discussed
structure, repeating unit, Bacillus, cell wall polysaccharide, Bacillus cereus
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, HF solvolysis, 31P NMR, MALDI-TOF MS, composition analysis, NMR-1D, mild hydrazinolysis Comments, role: HF-PS, and one of the residue of trisaccharide is substituted with ?DGlcp(1-?).
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