Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 18237723Publication DOI: 10.1016/j.carres.2008.01.003Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: G. Widmalm <gw
organ.su.se>
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, Sweden
The structure of the O-antigen polysaccharide (PS) from Escherichia coli O176 has been determined. Component analysis together with (1)H and (13)C NMR spectroscopy was employed to elucidate the structure. Inter-residue correlations were determined by (1)H, (1)H NOESY and (1)H, (13)C heteronuclear multiple-bond correlation experiments. The PS is composed of tetrasaccharide repeating units with the following structure: Cross-peaks of low intensity from α-linked mannopyranosyl residues were present in the (1)H, (1)H TOCSY NMR spectra and further analysis of these showed that they originate from the terminal part of the polysaccharide. Consequently, the biological repeating unit has a 3-substituted N-acetyl-d-galactosamine residue at its reducing end. The repeating unit of the E. coli O176 O-antigen is similar to those from E. coli O17 and O77, thereby explaining the reported cross-reactivities between the strains, and identical to that of Salmonella cerro (O:6, 14, 18)
Lipopolysaccharide, NMR, Escherichia coli, biological repeating unit, Salmonella cerro
Structure type: polymer chemical repeating unit
Location inside paper: p. 808
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_130648,IEDB_130701,IEDB_136104,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_140116,IEDB_141584,IEDB_141830,IEDB_142488,IEDB_143632,IEDB_144983,IEDB_146664,IEDB_152206,IEDB_885822,IEDB_983930,IEDB_983931,SB_136,SB_192,SB_196,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, sugar analysis, GLC, mild acid hydrolysis, NMR-1D
Related record ID(s): 22712, 23197
NCBI Taxonomy refs (TaxIDs): 2170725Reference(s) to other database(s): GTC:G21240NX, GlycomeDB:
37033
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There is only one chemically distinct structure:
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