CSDB: Search results

Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: diarrhea [ICD11: ME05.1

, ICD11: SA55

]; infection due to Campylobacter jejuni [ICD11: XN4Q5

]

NCBI PubMed ID: 19114545
Journal NLM ID: 0246127
Publisher: American Society for Microbiology
Correspondence: patricia.guerry

med.navy.mil
Institutions: Dept. of Chemistry, University of Guelph, Guelph, Ontario, Canada N1G 2W11, Dept. of Enteric Diseases, Naval Medical Research Center, Silver Spring, Maryland 209102, and Naval Medical Research Center Detachment, Lima, Peru3

The capsule polysaccharide (CPS) of Campylobacter jejuni is one of the few identified virulence determinants of this important human pathogen. Since CPS conjugate vaccines have been so effective against other mucosal pathogens, we evaluated this approach using CPSs from two strains of C. jejuni, 81-176 (HS23 and HS36 serotype complex) and CG8486 (HS4 serotype complex). The CPSs of 81-176 and CG8486 were independently linked to the carrier protein CRM197 by reductive amination between an aldehyde(s), strategically created at the nonreducing end of each CPS, and accessible amines of CRM197. In both cases, the CPS:CRM197 ratio used was 2:1 by weight. Mass spectrometry and gel electrophoresis showed that on average, each glycoconjugate preparation contained, at least in part, two to five CPSs attached to one CRM197. When administered subcutaneously to mice, these vaccines elicited robust immune responses and significantly reduced the disease following intranasal challenge with the homologous strains of C. jejuni. The CPS81-176-CRM197 vaccine also provided 100% protection against diarrhea in the New World monkey Aotus nancymaae following orogastric challenge with C. jejuni 81-176.

Campylobacter jejuni, capsule, polysaccharide conjugate vaccine

Structure type: oligomer
Location inside paper: p. 1129, fig. 1A
Compound class: CPS
Contained glycoepitopes: IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_141794,IEDB_141807,IEDB_151528,IEDB_151531,IEDB_190606,SB_173,SB_7

Methods: 1H NMR, GC-MS, ELISA, 31P NMR, MALDI-TOF MS, serological methods, statistical analysis
Biological activity: serological data, biological activity data

Related record ID(s): 23455
NCBI Taxonomy refs (TaxIDs): 354242
Show glycosyltransferases

There is only one chemically distinct structure:

CO[C@H]1[C@H](O[C@H]2O[C@H](CO)[C@H](O)[C@H](O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O[C@H]4O[C@H](CCO)[C@@H](O)[C@@H](OC)[C@@H]4O[C@H]4O[C@H](CO)[C@H](O)[C@H](O[C@@H]5O[C@H](CO)[C@@H](O)[C@H](O[C@H]6O[C@H](CCO)[C@@H](O)[C@@H](OC)[C@@H]6O[C@H]6O[C@H](CO)[C@H](O)[C@H](O[C@@H]7O[C@H](CO)[C@@H](O)[C@H](O[C@H]8O[C@H](CCO)[C@@H](O)[C@@H](OC)[C@@H]8O[C@H]8O[C@H](CO)[C@H](O)[C@H](O)[C@H]8OP(N)(=O)OC)[C@H]7NC(C)=O)[C@H]6OP(N)(=O)OC)[C@H]5NC(C)=O)[C@H]4OP(N)(=O)OC)[C@H]3NC(C)=O)[C@H]2OP(N)(=O)OC)[C@@H](O[C@H]2[C@H](O)[C@@H](CO)O[C@@H](O[C@H]3[C@@H](O)[C@@H](CO)O[C@H](O)[C@@H]3OP(N)(=O)OC)[C@@H]2NC(C)=O)O[C@H](CCO)[C@H]1O

SVG MW SMILES 3D mol Ionize

Warning = sub-repeat ending at aDGalp: unknown unit count was assumed to be 3