Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Burkholderia pseudomallei [ICD11:
XN3LD 
];
infection due to Pseudomonas pseudomallei [ICD11:
XN8AA ]
NCBI PubMed ID: 18558401Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: N. Parthasarathy <Narayanan.Parthasarathy
us.army.mil>
Institutions: Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, MD, USA
We developed a microarray platform by immobilizing bacterial 'signature' carbohydrates onto epoxide modified glass slides. The carbohydrate microarray platform was probed with sera from non-melioidosis and melioidosis (Burkholderia pseudomallei) individuals. The platform was also probed with sera from rabbits vaccinated with Bacillus anthracis spores and Francisella tularensis bacteria. By employing this microarray platform, we were able to detect and differentiate B. pseudomallei, B. anthracis and F. tularensis antibodies in infected patients, and infected or vaccinated animals. These antibodies were absent in the sera of nai ve test subjects. The advantages of the carbohydrate microarray technology over the traditional indirect hemagglutination and microagglutination tests for the serodiagnosis of melioidosis and tularemia are discussed. Furthermore, this array is a multiplex carbohydrate microarray for the detection of all three biothreat bacterial infections including melioidosis, anthrax and tularemia with one, multivalent device. The implication is that this technology could be expanded to include a wide array of infectious and biothreat agents
Bacillus anthracis (anthrax), Burkholderia pseudomallei (melioidosis), carbohydrate microarray, Francisella tularensis (tularemia), Serum antibodies
Structure type: polymer chemical repeating unit
Location inside paper: p. 2784, fig. 1A
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: serological methods
Biological activity: biological activity data
Related record ID(s): 23409, 23462, 23464, 23465, 23466
NCBI Taxonomy refs (TaxIDs): 28450Reference(s) to other database(s): GTC:G54867WL
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There is only one chemically distinct structure:
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(previously named: Pseudomonas pseudomallei)