Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: functional gastrointestinal disorders [ICD11:
DD9Y 
];
infection due to Salmonella enterica [ICD11:
XN5VC ]
The structure was elucidated in this paperNCBI PubMed ID: 19058946Publication DOI: 10.1016/j.jpba.2008.10.013Journal NLM ID: 8309336Publisher: London: Elsevier
Correspondence: Z. Kaczyński <zbyszek
chem.univ.gda.pl>
Institutions: Faculty of Chemistry, University of Gdansk, Gdansk, Poland
A neutral O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide (LPS) of Salmonella Abortusequi O4 bacterium (previously serogroup B). As determined by compositional analyses and NMR spectroscopy, the O-polysaccharide consists of four or five residues in the repeating subunit. The assigned structures are: and A distribution of the repeating units in O-chain was analysed by Western blotting with anti O12 serum and MS spectrometry of oligosaccharides obtained from partial hydrolysis of polysaccharide
NMR, structure, O-polysaccharide, Salmonella abortusequi
Structure type: suggested polymer biological repeating unit
Location inside paper: p. abstract, p. 681, fig. 3 (a)
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_127517,IEDB_130701,IEDB_135509,IEDB_135513,IEDB_135514,IEDB_135611,IEDB_136093,IEDB_136105,IEDB_136775,IEDB_136906,IEDB_137472,IEDB_137486,IEDB_141794,IEDB_144983,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_225177,IEDB_885823,IEDB_983930,SB_44,SB_67,SB_7,SB_72
Methods: 13C NMR, 1H NMR, GLC-MS, NMR-2D, FAB-MS, partial acid hydrolysis, SDS-PAGE, sugar analysis, GLC, Western blotting, composition analysis, NMR-1D, immunoblotting
Related record ID(s): 2157, 6380, 6552, 8628, 20089, 22467, 22845, 23457, 23735
NCBI Taxonomy refs (TaxIDs): 590Reference(s) to other database(s): GTC:G42214IC, GlycomeDB:
25593
Show glycosyltransferases
NMR conditions: in D2O at 343 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,4,3 aXAbep 101.7 64.8 34.4 70.6 68.1 16.9
3,4 aDManp 101.0 80.3 78.8 69.6 74.9 61.9
3 aLRhap 102.9 68.2 70.5 82.9 69.3 18.4
aDGalp 102.4 69.3 78.3 70.4 71.7 62.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,3 aXAbep 5.12 4.05 1.99 3.87 4.10 1.18
3,4 aDManp 5.29 4.03 4.08 3.88 3.97 3.84
3 aLRhap 5.09 4.10 4.00 3.58 3.95 1.33
aDGalp 5.19 3.95 3.97 4.08 4.08 3.75
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,4,3 aXAbep 101.7/5.12 64.8/4.05 34.4/1.99 70.6/3.87 68.1/4.10 16.9/1.18
3,4 aDManp 101.0/5.29 80.3/4.03 78.8/4.08 69.6/3.88 74.9/3.97 61.9/3.84
3 aLRhap 102.9/5.09 68.2/4.10 70.5/4.00 82.9/3.58 69.3/3.95 18.4/1.33
aDGalp 102.4/5.19 69.3/3.95 78.3/3.97 70.4/4.08 71.7/4.08 62.2/3.75
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,3 | aXAbep | 5.12 | 4.05 | 1.99 | 3.87 | 4.10 | 1.18 |
| 3,4 | aDManp | 5.29 | 4.03 | 4.08 | 3.88 | 3.97 | 3.84 |
| 3 | aLRhap | 5.09 | 4.10 | 4.00 | 3.58 | 3.95 | 1.33 |
| | aDGalp | 5.19 | 3.95 | 3.97 | 4.08 | 4.08 | 3.75 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,4,3 | aXAbep | 101.7 | 64.8 | 34.4 | 70.6 | 68.1 | 16.9 |
| 3,4 | aDManp | 101.0 | 80.3 | 78.8 | 69.6 | 74.9 | 61.9 |
| 3 | aLRhap | 102.9 | 68.2 | 70.5 | 82.9 | 69.3 | 18.4 |
| | aDGalp | 102.4 | 69.3 | 78.3 | 70.4 | 71.7 | 62.2 |
|
There is only one chemically distinct structure:
Found 
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(later renamed to: Salmonella enterica O4 ssp. enterica sv. Abortusequi)