The pathogenic mechanisms of Gram-negative infection in cystic fibrosis are only just beginning to be explored at the molecular level. Several virulence factors have been defined, one of the most important is the lipopolysaccharide molecule. In order to fully understand the mechanisms of bacterial infection and host recognition a full structure/activity study of lipopolysaccharide is needed. In the present paper, we define the complete structure of the O-specific polysaccharide from the lipopolysaccharide from Burkholderia anthina, an uncommon pathogen of cystic fibrosis patients

Lipopolysaccharide, structure, Bacterial, host, virulence, polysaccharide, Burkholderia, molecular, molecule, recognition, factor, infection, level, O-specific, O-specific polysaccharide, mechanism, defined, Gram-negative, pathogen, cystic fibrosis, virulence factor, PDF, order, pathogenic, virulence factors, Fibrosis, Paper

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
2,2	aLRhap	102.4	66.6	74.6	70.4	69.3	17.1
2	aLRhap	100.7	78.3	70.1	72.0	69.4	17.1
	aDGalp	94.6	74.3	69.3	69.6	71.0	60.9


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
2,2	aLRhap	4.87	4.15	3.75	3.47	3.64	1.18
2	aLRhap	5.09	3.95	3.76	3.37	3.63	1.18
	aDGalp	5.05	3.81	3.97	3.90	4.08	3.61


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
2,2	aLRhap	102.4/4.87	66.6/4.15	74.6/3.75	70.4/3.47	69.3/3.64	17.1/1.18
2	aLRhap	100.7/5.09	78.3/3.95	70.1/3.76	72.0/3.37	69.4/3.63	17.1/1.18
	aDGalp	94.6/5.05	74.3/3.81	69.3/3.97	69.6/3.90	71.0/4.08	60.9/3.61

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

*O[C@@H]1[C@@H](O)[C@H](C)O[C@@H](O[C@H]2[C@H](O[C@@H]3[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]3*)O[C@@H](C)[C@H](O)[C@H]2O)[C@@H]1O

454.425 g/mol (C₁₈H₃₀R₂O₁₃, R = next and previous repeats, not counted in mol weight)