The serotyping of O and H antigens is an important first step in the characterization of Salmonella enterica. However, serotyping has become increasingly technically demanding and expensive to perform. We have therefore sequenced additional S. enterica O antigen gene clusters to provide information for the development of DNA-based serotyping methods. Three S. enterica isolates had O antigen gene clusters with homology to the Escherichia coli O123 O antigen region. O antigen clusters from two serogroup O58 S. enterica strains had approximately 85 % identity with the E. coli O123 O antigen region over their entire length, suggesting that these Salmonella and E. coli O antigen regions evolved from a common ancestor. The O antigen cluster of a Salmonella serogroup O41 isolate had a lower level of identity with E. coli O123 over only part of its O antigen DNA cluster sequence, suggesting a different and more complex evolution of this gene cluster than those in the O58 strains. A large part of the Salmonella O41 O antigen DNA cluster had very close identity with the O antigen cluster of an O62 strain. This region of DNA homology included the wzx and wzy genes. Therefore, molecular serotyping tests using only the O41 or O62 wzx and wzy genes would not differentiate between the two serogroups. The E. coli O123 O-antigenic polysaccharide and its repeating unit were characterized, and the chemical structure for E. coli O123 was entirely consistent with the O antigen gene cluster sequences of E. coli O123 and the Salmonella O58 isolates. An understanding of both the genetic and structural composition of Salmonella and E. coli O antigens is necessary for the development of novel molecular methods for serotyping these organisms

structure, O-antigen, serogroup, Salmonella enterica, serotyping

← originally published structure   Show legend

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
3,3,6,4,2	lS3HOBut	174.50	45.22	65.89	22.80
3,3,6,4	xDAla?	175.21	50.56	16.88
3,3,6	bDQuip4N	103.35	73.24	76.81	57.63	72.01	17.34
3,3,2	Ac	173.97	23.02
3,3	aDGlcpN	97.98	54.37	71.82	71.28	69.89	68.52
3,2	Ac	174.98	22.96
3	aLQuipN	97.29	54.50	76.33	74.48	68.46	17.32
2	Ac	174.12	23.02
6	30%Ac
	aDGlcpN	97.29	54.06	75.44	68.64	72.35	60.98


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
3,3,6,4,2	lS3HOBut	-	2.42	4.18	1.23
3,3,6,4	xDAla?	-	4.22	1.26
3,3,6	bDQuip4N	4.46	3.46	3.79	3.80	3.55	1.18
3,3,2	Ac	-	1.96
3,3	aDGlcpN	5.03	3.91	3.71	4.24	3.70	3.90-4.08
3,2	Ac	-	2.04
3	aLQuipN	4.95	4.07	3.79	3.28	4.26	1.25
2	Ac	-	1.95
6	30%Ac
	aDGlcpN	4.99	4.04	3.87	3.59	4.25	3.80-3.82


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
3,3,6,4,2	lS3HOBut		45.22/2.42	65.89/4.18	22.80/1.23
3,3,6,4	xDAla?		50.56/4.22	16.88/1.26
3,3,6	bDQuip4N	103.35/4.46	73.24/3.46	76.81/3.79	57.63/3.80	72.01/3.55	17.34/1.18
3,3,2	Ac		23.02/1.96
3,3	aDGlcpN	97.98/5.03	54.37/3.91	71.82/3.71	71.28/4.24	69.89/3.70	68.52/3.90-4.08
3,2	Ac		22.96/2.04
3	aLQuipN	97.29/4.95	54.50/4.07	76.33/3.79	74.48/3.28	68.46/4.26	17.32/1.25
2	Ac		23.02/1.95
6	30%Ac
	aDGlcpN	97.29/4.99	54.06/4.04	75.44/3.87	68.64/3.59	72.35/4.25	60.98/3.80-3.82

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

*O[C@H]1[C@H](NC(=O)[C@@H](C)NC(=O)C[C@H](C)O)[C@@H](C)O[C@@H](OC[C@H]2O[C@H](O[C@@H]3[C@@H](O)[C@H](C)O[C@@H](O[C@@H]4[C@@H](NC(C)=O)[C@@H](*)O[C@H](COC(C)=O)[C@H]4O)[C@H]3NC(C)=O)[C@H](NC(C)=O)[C@@H](O)[C@@H]2O)[C@@H]1O

937.947 g/mol (C₃₉H₆₃R₂N₅O₂₁, R = next and previous repeats, not counted in mol weight)