Engskog MK, Yildirim HH, Li J, Richards JC, Deadman M, Hood DW, Schweda EK A dual role for the lex2 locus: identification of galactosyltransferase activity in non-typeable Haemophilus influenzae strains 1124 and 2019 Carbohydrate Research344(5) (2009)
632-641
NCBI PubMed ID:19211098 Publication DOI:10.1016/j.carres.2009.01.005 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: Elke.Schwedaki.se (E. K. H. Schweda) Institutions: Clinical Research Centre, Karolinska Institutet and Sodertorn University, NOVUM, S-141 86 Huddinge, Sweden
Lipopolysaccharide (LPS) of Haemophilus influenzae comprises a conserved tri-l-glycero-d-manno-heptosyl inner-core moiety (L-α-D-Hepp-(1→2)-[PEtn→6]-L-α-D-Hepp-(1→3)-[β-D-GlcIp-(1→4)]-L-α-D-Hepp-(1→5)-α-Kdop) to which addition of β-D-Glcp to O-4 of GlcI in serotype b strains is controlled by the gene lex2B. In non-typeable H. influenzae strains 1124 and 2019, however, a β-D-Galp is linked to O-4 of GlcI. In order to test the hypothesis that the lex2 locus is involved in the expression of β-D-Galp-(1→4-β-D-Glcp-(1→ from HepI, lex2B was inactivated in strains 1124 and 2019, and LPS glycoform populations from the resulting mutant strains were investigated. Detailed structural analyses using NMR techniques and electrospray-ionisation mass spectrometry (ESIMS) on O-deacylated LPS and core oligosaccharide material (OS), as well as ESIMS(n) on permethylated dephosphorylated OS, indicated both lex2B mutant strains to express only β-D-Glcp extensions from HepI. This provides strong evidence that Lex2B functions as a galactosyltransferase adding a β-D-Galp to O-4 of GlcI in these strains, indicating that allelic polymorphisms in the lex2B sequence direct alternative functions of the gene product
Related record ID(s): 23992, 23993, 23994, 23995, 23996 NCBI Taxonomy refs (TaxIDs):1232659 Reference(s) to other database(s): GTC:G81753HX Show glycosyltransferases
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ki.se (E. K. H. Schweda)