Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: salmonellosis [ICD11:
1A09 
, ICD11:
XN0QE ];
infection due to Salmonella enterica [ICD11:
XN5VC ]
The structure was elucidated in this paperNCBI PubMed ID: 19394591Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Z. Kaczynski <zbyszek
chem.univ.gda.pl>
Institutions: Faculty of Chemistry, University of Gdansk, Gdansk, Poland
The O-antigenic polysaccharide of Salmonella Mara O:39 (formerly Q) was investigated by sugar and methylation analyses, absolute configuration assignment, mass spectrometry and NMR spectroscopy. The experiments revealed an O-polysaccharide chain composed of the following linear tetrasaccharide repeating units with the structure: →2)-α-L-Quip3NAc-(1→3)-α-D-Manp-(1→3)-α-L-Fucp-(1→3)-α-D-GalpNAc-(1→ where α-L-Quip3NAc is the residue of 3-acetamido-3,6-dideoxy-α-L-glucopyranose. This repeating unit is the first published structure of the O-polysaccharide from 27 serotypes of Salmonella bacteria belonging to serogroup O:39 in the Kauffmann-White classification system
NMR, O-antigen, 3-Acetamido-3, 6-dideoxy-L-glucose, Salmonella Mara, serogroup O:39
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.1054, p.1056
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_130701,IEDB_136045,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_142489,IEDB_144562,IEDB_144983,IEDB_152206,IEDB_152214,IEDB_174333,IEDB_885822,IEDB_983930,SB_44,SB_67,SB_72,SB_86
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, sugar analysis, mild acid hydrolysis, NMR-1D
NCBI Taxonomy refs (TaxIDs): 59201Reference(s) to other database(s): GTC:G98777XJ
Show glycosyltransferases
NMR conditions: in D2O at 313 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,3,3 Ac 176.07 23.48
3,3,3 aLQuip3N 96.19 73.28 53.21 73.81 69.90 17.90
3,3 aDManp 102.88 71.08 76.21 68.68 74.59 62.04
3 aLFucp 102.40 68.45 78.35 72.71 68.26 16.50
2 Ac 176.12 23.68
aDGalpN 95.10 49.40 75.91 69.91 72.74 62.04
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,3,3 Ac - 2.06
3,3,3 aLQuip3N 5.58 3.70 4.26 3.30 3.94 1.30
3,3 aDManp 5.13 4.22 4.09 3.79 3.81 3.70-3.90
3 aLFucp 4.96 3.86 4.01 3.97 4.12 1.19
2 Ac - 2.08
aDGalpN 5.19 4.48 3.75 4.05 3.85 3.70-3.90
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,3,3 Ac 23.48/2.06
3,3,3 aLQuip3N 96.19/5.58 73.28/3.70 53.21/4.26 73.81/3.30 69.90/3.94 17.90/1.30
3,3 aDManp 102.88/5.13 71.08/4.22 76.21/4.09 68.68/3.79 74.59/3.81 62.04/3.70-3.90
3 aLFucp 102.40/4.96 68.45/3.86 78.35/4.01 72.71/3.97 68.26/4.12 16.50/1.19
2 Ac 23.68/2.08
aDGalpN 95.10/5.19 49.40/4.48 75.91/3.75 69.91/4.05 72.74/3.85 62.04/3.70-3.90
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,3,3 | Ac |
| 2.06 | |
| 3,3,3 | aLQuip3N | 5.58 | 3.70 | 4.26 | 3.30 | 3.94 | 1.30 |
| 3,3 | aDManp | 5.13 | 4.22 | 4.09 | 3.79 | 3.81 | 3.70
3.90 |
| 3 | aLFucp | 4.96 | 3.86 | 4.01 | 3.97 | 4.12 | 1.19 |
| 2 | Ac |
| 2.08 | |
| | aDGalpN | 5.19 | 4.48 | 3.75 | 4.05 | 3.85 | 3.70
3.90 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,3,3 | Ac | 176.07 | 23.48 | |
| 3,3,3 | aLQuip3N | 96.19 | 73.28 | 53.21 | 73.81 | 69.90 | 17.90 |
| 3,3 | aDManp | 102.88 | 71.08 | 76.21 | 68.68 | 74.59 | 62.04 |
| 3 | aLFucp | 102.40 | 68.45 | 78.35 | 72.71 | 68.26 | 16.50 |
| 2 | Ac | 176.12 | 23.68 | |
| | aDGalpN | 95.10 | 49.40 | 75.91 | 69.91 | 72.74 | 62.04 |
|
There is only one chemically distinct structure:
Found 
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(previously named: Salmonella mara O39)