Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Host organism: Bos taurus; Homo sapiens
Associated disease: anthrax [ICD11:
1B97 
, ICD11:
XN94F ];
pneumonia [ICD11:
CA40 ];
infection due to Bacillus anthracis [ICD11:
XN94F ]
NCBI PubMed ID: 19270075Publication DOI: 10.1093/glycob/cwp036Journal NLM ID: 9104124Publisher: IRL Press at Oxford University Press
Correspondence: rcarlson
ccrc.uga.edu
Institutions: Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, Centers for Disease Control and Prevention, 1600 Clifton Rd., MS D-11, Atlanta, GA 30333, USA, Departments of Microbiology and Biotechnology, University of Tübingen, D-72076 Tübingen, Germany
The immunoreactivities of hydrogen fluoride- (HF) released cell wall polysaccharides (HF-PSs) from selected Bacillus anthracis and Bacillus cereus strains were compared using antisera against live and killed B. anthracis spores. These antisera bound to the HF-PSs from B. anthracis and from three clinical B. cereus isolates (G9241, 03BB87 and 03BB102) obtained from cases of severe or fatal human pneumonia but did not bind the HF-PSs from the closely related B. cereus ATCC 10987 or from B. cereus type strain ATCC 14579. Antiserum against a keyhole limpet hemocyanin conjugate of the B. anthracis HF-PS (HF-PS-KLH) also bound HF-PSs and cell walls from B. anthracis and the three clinical B. cereus isolates, and B. anthracis spores. These results indicate that the B. anthracis HF-PS is an antigen in both B. anthracis cell walls and spores, and that it shares cross-reactive, and possibly pathogenicity-related, epitopes with three clinical B. cereus isolates that caused severe disease. The anti-HF-PS-KLH antiserum cross-reacted with the bovine serum albumin (BSA)-conjugates of all B. anthracis and all B. cereus HF-PSs tested, including those from non-clinical B. cereus ATCC 10987 and ATCC 14579 strains. Finally, the serum of vaccinated (anthrax vaccine adsorbed [AVA]) Rhesus macaques that survived inhalation anthrax contained IgG antibodies that bound the B. anthracis HF-PS-KLH conjugate. These data indicate that HF-PSs from the cell walls of the bacilli tested here are (i) antigens that contain (ii) a potentially virulence-associated carbohydrate antigen motif, and (iii) another antigenic determinant that is common to B. cereus strains.
antigens, specificity, polysaccharides, Bacillus anthracis, Bacillus cereus, polysaccharides specificity
Structure type: polymer chemical repeating unit
Location inside paper: p.666, fig.1
Trivial name: HF-PS
Compound class: cell wall polysaccharide
Contained glycoepitopes: IEDB_135813,IEDB_136044,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_140108,IEDB_141794,IEDB_141807,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_885813,SB_165,SB_166,SB_187,SB_195,SB_30,SB_7,SB_88
Methods: HF solvolysis, ELISA, serological methods
Biological activity: serological data, immunoreactivity data
Comments, role: repeating unit of the HF-released polysaccharide, as previously reported; this structure was revised later
Related record ID(s): 24079
NCBI Taxonomy refs (TaxIDs): 198094,
260799,
1392Reference(s) to other database(s): GTC:G17290CI, GlycomeDB:
37286
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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