Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: urethritis [ICD11:
GC02 
];
endocarditis [ICD11:
BB40 , ICD11:
BB4Z ]
The structure was elucidated in this paperNCBI PubMed ID: 19270085Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: dcstein
umd.edu
Institutions: Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
Neisseria sicca 4320 expresses two carbohydrate-containing components with sodium dodecyl sulfate-polyacrylamide gel electrophoresis mobilities that resemble those of lipooligosaccharide and lipopolysaccharide. Using matrix-assisted laser desorption ionization--time of flight and electrospray ionization mass spectrometry, we characterized a disaccharide carbohydrate repeating unit expressed by this strain. Gas chromatography identified the sugars composing the unit as rhamnose and N-acetyl-D-glucosamine. Glycosidase digestion confirmed the identity of the nonreducing terminal sugar of the disaccharide and established its β-anomeric configuration. Mass spectrometry analysis and lectin binding were used to verify the linkages within the disaccharide repeat. The results revealed that the disaccharide repeat is [-4) β-L-rhamnose (1-3) β-N-acetyl-D-glucosamine (1-] with an N-acetyl-D-glucosamine nonreducing terminus. This work is the first structural characterization of a molecule that possesses rhamnose in the genus Neisseria.
Lipopolysaccharide, Lipooligosaccharide, ionization mass spectrometry, linkage, lectin, laser desorption, Neisseria sicca
Structure type: polymer chemical repeating unit
Location inside paper: abstract
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_225177,IEDB_885823,IEDB_983931,SB_192,SB_61
Methods: GC-MS, de-O-acylation, SDS-PAGE, ESI-MS, Western blotting, MALDI-TOF MS, composition analysis
NCBI Taxonomy refs (TaxIDs): 933266Reference(s) to other database(s): GTC:G12854AK, GlycomeDB:
37292
Show glycosyltransferases
There is only one chemically distinct structure:
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