Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: urinary tract infections (UTI) [ICD11:
GC08 
]
The structure was elucidated in this paperJournal NLM ID: 9420101WWW link: http://www.maik.ru/abstract/biochem/9/biochem3_9p370abs.htmPublisher: Springer Science and Business Media
Correspondence: olga.ovchinnikova
gmail.com
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 147, Moscow, 119991 Russia
Acidic O-specific polysaccharide containing D-glucose, D-glucuronic acid, L-fucose, and 2-acetamido-2-deoxy-D-glucose was obtained by mild acid degradation of lipopolysaccharide from Providencia alcalifaciens O46. The following structure of the hexasaccharide repeating unit of the O-specific polysaccharide was established using methylation analysis along with1H and13C NMR spectroscopy, including 2D1H,1H-COSY,TOCSY, ROESY,1H,13C-HSQC, and HMQC-TOCSY experiments: [carbohydrate structure: see text].
structure, O-antigen, O-specific polysaccharide, Providencia alcalifaciens, NMR spectrocscopy
Structure type: polymer chemical repeating unit
Location inside paper: p.370, abstract
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115136,IEDB_136045,IEDB_137340,IEDB_140630,IEDB_141807,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_152214,IEDB_174333,IEDB_423153,IEDB_983931,SB_192,SB_86
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, sugar analysis, GLC, de-O-acetylation
NCBI Taxonomy refs (TaxIDs): 126385Reference(s) to other database(s): GTC:G17497EO
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,2,4,4 bDGlcpA 104.9 73.9 83.6 73.7 78.8 175.4
3,2,4,3 aDGlcp 101.3 73.5 74.4 71.8 73.5 62.0
3,2,4 aLFucp 101.9 70.5 75.6 81.4 69.1 16.7
3,2 aLFucp 101.0 69.4 70.3 81.5 68.5 16.7
3 bDGlcp 101.6 79.8 78.1 71.1 77.2 62.1
2 Ac 176.8 23.4
6 80%Ac
aDGlcpN 99.3 54.9 77.5 69.6 73.1 61.5
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,2,4,4 bDGlcpA 4.58 3.49 3.57 3.80 3.65 -
3,2,4,3 aDGlcp 5.28 3.92 4.12 3.58 4.08 3.81-3.85
3,2,4 aLFucp 4.98 4.06 4.07 4.13 4.58 1.28
3,2 aLFucp 5.26 3.85 3.76 3.76 4.27 1.30
3 bDGlcp 4.67 3.45 3.72 3.43 3.45 3.74-3.93
2 Ac - 2.08
6 80%Ac
aDGlcpN 5.17 3.92 4.12 3.58 4.08 3.81-3.85
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,2,4,4 bDGlcpA 104.9/4.58 73.9/3.49 83.6/3.57 73.7/3.80 78.8/3.65
3,2,4,3 aDGlcp 101.3/5.28 73.5/3.92 74.4/4.12 71.8/3.58 73.5/4.08 62.0/3.81-3.85
3,2,4 aLFucp 101.9/4.98 70.5/4.06 75.6/4.07 81.4/4.13 69.1/4.58 16.7/1.28
3,2 aLFucp 101.0/5.26 69.4/3.85 70.3/3.76 81.5/3.76 68.5/4.27 16.7/1.30
3 bDGlcp 101.6/4.67 79.8/3.45 78.1/3.72 71.1/3.43 77.2/3.45 62.1/3.74-3.93
2 Ac 23.4/2.08
6 80%Ac
aDGlcpN 99.3/5.17 54.9/3.92 77.5/4.12 69.6/3.58 73.1/4.08 61.5/3.81-3.85
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,2,4,4 | bDGlcpA | 4.58 | 3.49 | 3.57 | 3.80 | 3.65 |
|
| 3,2,4,3 | aDGlcp | 5.28 | 3.92 | 4.12 | 3.58 | 4.08 | 3.81
3.85 |
| 3,2,4 | aLFucp | 4.98 | 4.06 | 4.07 | 4.13 | 4.58 | 1.28 |
| 3,2 | aLFucp | 5.26 | 3.85 | 3.76 | 3.76 | 4.27 | 1.30 |
| 3 | bDGlcp | 4.67 | 3.45 | 3.72 | 3.43 | 3.45 | 3.74
3.93 |
| 2 | Ac |
| 2.08 | |
| 6 | 80%Ac | |
| | aDGlcpN | 5.17 | 3.92 | 4.12 | 3.58 | 4.08 | 3.81
3.85 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,2,4,4 | bDGlcpA | 104.9 | 73.9 | 83.6 | 73.7 | 78.8 | 175.4 |
| 3,2,4,3 | aDGlcp | 101.3 | 73.5 | 74.4 | 71.8 | 73.5 | 62.0 |
| 3,2,4 | aLFucp | 101.9 | 70.5 | 75.6 | 81.4 | 69.1 | 16.7 |
| 3,2 | aLFucp | 101.0 | 69.4 | 70.3 | 81.5 | 68.5 | 16.7 |
| 3 | bDGlcp | 101.6 | 79.8 | 78.1 | 71.1 | 77.2 | 62.1 |
| 2 | Ac | 176.8 | 23.4 | |
| 6 | 80%Ac | |
| | aDGlcpN | 99.3 | 54.9 | 77.5 | 69.6 | 73.1 | 61.5 |
|
There is only one chemically distinct structure:
Found 
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