Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: dysentery [ICD11:
SA56 
];
bacillary dysentery (shigellosis) [ICD11:
1A02 , ICD11:
SA56 , ICD11:
XN7HG ];
infection due to Shigella dysenteriae [ICD11:
XN285 ]
The structure was elucidated in this paperPublication DOI: 10.1134/S1068162009010178Journal NLM ID: 9420101Publisher: Springer Science and Business Media
Correspondence: perepel
ioc.ac.ru
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 147, Moscow, 119991 Russia
The structure of the O-specific polysaccharide from Shigella dysenteriae type 10, which has been reported previously in Bioorganic chemistry (1977, vol.3, pp. 1219-1225), is refined: [formula, see text].
structure, O-antigen, NMR spectroscopy, Shigella dysenteriae
Structure type: polymer chemical repeating unit
Location inside paper: p.131, Table 1, Table 2
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_137340,IEDB_137485,IEDB_141807,IEDB_144983,IEDB_149549,IEDB_151531,IEDB_152206,IEDB_225177,IEDB_885823,IEDB_983930,SB_44,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, NMR-1D, mild acid degradation
Comments, role: OPS was obtained by a soft acid degradation of the LPS
Related record ID(s): 8898, 21528, 108637, 110362, 115245
NCBI Taxonomy refs (TaxIDs): 622Reference(s) to other database(s): GTC:G59746QG, GlycomeDB:
3523
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4,3,3 bDManp 97.8 78.7 74.5 68.4 77.8 61.6
4,3,2 Ac 175.5-176.3 23.4
4,3 aDManpN 96.5 50.4 76.1 66.0 74.0 61.5
4 bLRhap 102.2 68.0 77.9 71.3 73.7 18.2
2 Ac 175.5-176.3 23.4
aDGlcpN 99.5 55.4 71.3 78.7 71.3 62.3
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4,3,3 bDManp 4.82 3.89 3.73 3.67 3.43 3.77-3.95
4,3,2 Ac - 2.03-2.07
4,3 aDManpN 5.06 4.62 4.24 3.61 3.97 3.81-3.86
4 bLRhap 4.88 4.30 3.67 3.46 3.46 1.34
2 Ac - 2.03-2.07
aDGlcpN 5.09 3.84 4.01 3.75 4.14 3.76-3.95
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4,3,3 bDManp 97.8/4.82 78.7/3.89 74.5/3.73 68.4/3.67 77.8/3.43 61.6/3.77-3.95
4,3,2 Ac 23.4/2.03-2.07
4,3 aDManpN 96.5/5.06 50.4/4.62 76.1/4.24 66.0/3.61 74.0/3.97 61.5/3.81-3.86
4 bLRhap 102.2/4.88 68.0/4.30 77.9/3.67 71.3/3.46 73.7/3.46 18.2/1.34
2 Ac 23.4/2.03-2.07
aDGlcpN 99.5/5.09 55.4/3.84 71.3/4.01 78.7/3.75 71.3/4.14 62.3/3.76-3.95
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4,3,3 | bDManp | 4.82 | 3.89 | 3.73 | 3.67 | 3.43 | 3.77
3.95 |
| 4,3,2 | Ac |
| 2.03
2.07 | |
| 4,3 | aDManpN | 5.06 | 4.62 | 4.24 | 3.61 | 3.97 | 3.81
3.86 |
| 4 | bLRhap | 4.88 | 4.30 | 3.67 | 3.46 | 3.46 | 1.34 |
| 2 | Ac |
| 2.03
2.07 | |
| | aDGlcpN | 5.09 | 3.84 | 4.01 | 3.75 | 4.14 | 3.76
3.95 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4,3,3 | bDManp | 97.8 | 78.7 | 74.5 | 68.4 | 77.8 | 61.6 |
| 4,3,2 | Ac | 175.5
176.3 | 23.4 | |
| 4,3 | aDManpN | 96.5 | 50.4 | 76.1 | 66.0 | 74.0 | 61.5 |
| 4 | bLRhap | 102.2 | 68.0 | 77.9 | 71.3 | 73.7 | 18.2 |
| 2 | Ac | 175.5
176.3 | 23.4 | |
| | aDGlcpN | 99.5 | 55.4 | 71.3 | 78.7 | 71.3 | 62.3 |
|
There is only one chemically distinct structure:
Found 
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