Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: diarrhea [ICD11:
ME05.1 
, ICD11:
SA55 ];
septicemia [ICD11:
MA15.Y ]
Publication DOI: 10.1002/ejoc.200801200Journal NLM ID: 9805750Publisher: Wiley-VCH
Correspondence: corsaro
unina.it
Institutions: Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy, Fax: +39-081-674393, Departamento Microbiología, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain
Plesiomonas shigelloides is a Gram-negative pathogenic bacterium belonging to the Enterobacteriaceae family. To date, only few lipopolysaccharide (LPS) structures from P. shigelloides strains are known. In particular, three core oligosaccharides have been found. Recently, we elucidated the structure of the O-antigen of P. shigelloides 302-73 (serotype O1) and in this paper we present the characterization of the core structure from the LPS of the same strain. The LPS was hydrolyzed under both alkaline and mildly acidic conditions. In both cases, a mixture of oligosaccharides was obtained, which was purified by gel filtration and HPAEC. The oligosaccharides were characterized by chemical analysis, 2D NMR spectroscopy and MALDI-TOF mass spectrometry. A new core structure was found for P. shigelloides. In particular, from the analysis of the acid hydrolysed product it was possible to reveal the presence of a of D-glycero-D-talo-2-octulopyranosonic acid (Ko) residue, which substitutes in part the terminal 3-doxy-D-manno-oct-2-ulosonic acid (Kdo) unit. The Ko residue is not frequently found in core structures.
Oligosaccharides, NMR spectroscopy, structure elucidation, sequence determination
Structure type: polymer chemical repeating unit
Location inside paper: p.1365
Compound class: O-polysaccharide, O-antigen
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, de-O-acylation, SDS-PAGE, sugar analysis, acid hydrolysis, alkaline degradation, MALDI-TOF MS, NMR-1D, HPAEC-PAD
Comments, role: ring sizes were assumed
Related record ID(s): 24176, 24177, 24178
NCBI Taxonomy refs (TaxIDs): 1315976
Show glycosyltransferases
There is only one chemically distinct structure:
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