Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: diarrhea [ICD11:
ME05.1 
, ICD11:
SA55 ];
cholera [ICD11:
1A00 , ICD11:
XN7N1 ]
NCBI PubMed ID: 19563890Journal NLM ID: 8406899Publisher: Elsevier
Correspondence: E.T. Ryan <etryan
partners.org>; julianne.rollenhagen
med.navy.mil
Institutions: Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114, USA
Antibodies specific for Vibrio cholerae lipopolysaccaride (LPS) are common in humans recovering from cholera, and constitute a primary component of the vibriocidal response, a serum complement-mediated bacteriocidal response correlated with protection against cholera. In order to determine whether transcutaneous immunization (TCI) with a V. cholerae neoglycoconjugate (CHO-BSA) comprised of a synthetic terminal hexasaccharide of the O-specific polysaccharide of V. cholerae O1 (Ogawa) conjugated with bovine serum albumin (BSA) could induce anti-V. cholerae LPS and vibriocidal responses, we applied CHO-BSA transcutaneously in the presence or absence of the immune adjuvant cholera toxin (CT) to mice. Transcutaneously applied neoglycoconjugate elicited prominent V. cholerae specific LPS IgG responses in the presence of CT, but not IgM or IgA responses. CT applied on the skin induced strong IgG and IgA serum responses. TCI with neoglycoconjugate did not elicit detectable vibriocidal responses, protection in a mouse challenge assay, or stool anti-V. cholerae IgA responses, irrespective of the presence or absence of CT. Our results suggest that transcutaneously applied synthetic V. cholerae neoglycoconjugate is safe and immunogenic, but predominantly induces systemic LPS responses of the IgG isotype.
conjugate, cholera, transcutaneous immunization
Structure type: oligomer
Location inside paper: p.4918, fig.1
Aglycon: spacer-BSA
Trivial name: Ogawa neoglycoconjugate
Compound class: neoglycoconjugate
Contained glycoepitopes: IEDB_130664,IEDB_161054,IEDB_161056,IEDB_161058
Methods: serological methods, statistical analysis, immunization
Biological activity: serological data
Synthetic data: chemical
NCBI Taxonomy refs (TaxIDs): 127906
Show glycosyltransferases
There is only one chemically distinct structure:
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