Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: bacillary dysentery [ICD11:
1A02 
, ICD11:
XN7HG ];
infection due to Shigella flexneri [ICD11:
XN7Y2 ]
NCBI PubMed ID: 9305766Publication DOI: 10.1016/S0378-1119(97)00144-3Journal NLM ID: 7706761Publisher: Amsterdam: Elsevier
Institutions: Division of Biochemistry and Molecular Biology, School of Life Sciences, The Australian National University, Canberra
With lysogeny by bacteriophage SfV, Shigella flexneri serotype Y is converted to serotype 5a. The glucosyl transferase gene (gtr) from bacteriophage SfV of S. flexneri, involved in serotype-specific conversion, was cloned and characterized. The DNA sequence of a 3.7 kb EcoRI-BamHI fragment of bacteriophage SfV which includes the gtr gene was determined. This gene, encoding a polypeptide of 417 aa with 47.67 kDa molecular mass, caused partial serotype conversion of S. flexneri from serotype Y to type V antigen as demonstrated by Western blotting and the sensitivity of the hybrid strain to phage Sf6. The deduced protein of the partially sequenced open reading frame upstream of the gtr showed similarity to various glycosyl transferases of other bacteria. Orf3, separated from the gtr by a non-coding region and transcribed convergently, codes for a 167 aa (18.8 kDa) protein found to have homology with tail fibre genes of phage lambda and P2
Shigella flexneri, Bacteriophage SfV, Glucosyl transferase gene, Phage conversion
Structure type: polymer chemical repeating unit
Location inside paper: p.208, fig 1, 1b
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_125613,IEDB_125614,IEDB_127514,IEDB_130422,IEDB_133752,IEDB_133753,IEDB_133754,IEDB_135813,IEDB_135849,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_141815,IEDB_141816,IEDB_142488,IEDB_143253,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_153213,IEDB_225177,IEDB_885823,IEDB_983931,SB_192
Methods: Western blotting, serological methods, genetic methods
Biological activity: serological data
Biosynthesis and genetic data: genetic data
Related record ID(s): 23609, 23908, 23909, 23910, 23911, 23912, 23914, 23915, 23916, 23917, 23918, 23982
NCBI Taxonomy refs (TaxIDs): 41434Reference(s) to other database(s): GTC:G95547PL, GlycomeDB:
37317
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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