Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: meningitis [ICD11:
1D01 
];
epiglottitis [ICD11:
CA06.1 ];
acute otitis media [ICD11:
AB00 , Life stage: adult];
infection due to Haemophilus influenzae [ICD11:
XN1P6 ]
The structure was elucidated in this paperNCBI PubMed ID: 19211098Publication DOI: 10.1016/j.carres.2009.01.005Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Elke.Schweda
ki.se (E. K. H. Schweda)
Institutions: Clinical Research Centre, Karolinska Institutet and Sodertorn University, NOVUM, S-141 86 Huddinge, Sweden
Lipopolysaccharide (LPS) of Haemophilus influenzae comprises a conserved tri-l-glycero-d-manno-heptosyl inner-core moiety (L-α-D-Hepp-(1→2)-[PEtn→6]-L-α-D-Hepp-(1→3)-[β-D-GlcIp-(1→4)]-L-α-D-Hepp-(1→5)-α-Kdop) to which addition of β-D-Glcp to O-4 of GlcI in serotype b strains is controlled by the gene lex2B. In non-typeable H. influenzae strains 1124 and 2019, however, a β-D-Galp is linked to O-4 of GlcI. In order to test the hypothesis that the lex2 locus is involved in the expression of β-D-Galp-(1→4-β-D-Glcp-(1→ from HepI, lex2B was inactivated in strains 1124 and 2019, and LPS glycoform populations from the resulting mutant strains were investigated. Detailed structural analyses using NMR techniques and electrospray-ionisation mass spectrometry (ESIMS) on O-deacylated LPS and core oligosaccharide material (OS), as well as ESIMS(n) on permethylated dephosphorylated OS, indicated both lex2B mutant strains to express only β-D-Glcp extensions from HepI. This provides strong evidence that Lex2B functions as a galactosyltransferase adding a β-D-Galp to O-4 of GlcI in these strains, indicating that allelic polymorphisms in the lex2B sequence direct alternative functions of the gene product
Lipopolysaccharide, Haemophilus influenzae, galactosyltransferase, structure analysis, lex2
Structure type: oligomer
Location inside paper: p.635, table 1, Hex2
Trivial name: glycoform core oligosaccharide
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_115009,IEDB_116046,IEDB_120354,IEDB_123890,IEDB_130650,IEDB_137777,IEDB_137779,IEDB_138949,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_140624,IEDB_142488,IEDB_146664,IEDB_2189047,IEDB_241118,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, sugar analysis, dephosphorylation, ESI-MS, mild acid hydrolysis, de-O-acylation with hydrazine, NMR-1D, CE-ESI-MS
Related record ID(s): 23693, 23992, 23994, 23995, 23996
NCBI Taxonomy refs (TaxIDs): 727
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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